ESR2 ELISA 试剂盒
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- 抗原 See all ESR2 ELISA试剂盒
- ESR2 (Estrogen Receptor 2 (ESR2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Estrogen Receptor Beta (ERb). No significant cross-reactivity or interference between Estrogen Receptor Beta (ERb) and analogues was observed.
- 灵敏度
- 0.117 ng/mL
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Estrogen Receptor 2 (ESR2) ELISA Kit
ESR2 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Estrogen Receptor 2 (ESR2) ELISA KitESR2 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Estrogen Receptor 2 (ESR2) ELISA KitESR2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Estrogen Receptor 2 (ESR2) ELISA KitESR2 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Estrogen Receptor 2 (ESR2) ELISA KitESR2 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Beta (ERb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen Receptor Beta (ERb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Receptor Beta (ERb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen Receptor Beta (ERb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all ESR2 ELISA试剂盒
- ESR2 (Estrogen Receptor 2 (ESR2))
- 别名
- Estrogen Receptor Beta (ESR2 产品)
- 别名
- ER-BETA ELISA Kit, ESR-BETA ELISA Kit, ESRB ELISA Kit, ESTRB ELISA Kit, Erb ELISA Kit, NR3A2 ELISA Kit, ER[b]1 ELISA Kit, zfER-beta1 ELISA Kit, zfER[b]1 ELISA Kit, erb2 ELISA Kit, CERB ELISA Kit, ERB ELISA Kit, erb ELISA Kit, esrb ELISA Kit, estrb ELISA Kit, nr3a2 ELISA Kit, ERbeta ELISA Kit, er-beta ELISA Kit, esr-beta ELISA Kit, ESR2 ELISA Kit, ERBETA ELISA Kit, er ELISA Kit, ER-beta ELISA Kit, ER[b] ELISA Kit, Estrb ELISA Kit, ER[b]2 ELISA Kit, ER[b]a ELISA Kit, zfER[b]2 ELISA Kit, zfER-beta2 ELISA Kit, Erb2 ELISA Kit, esr2 ELISA Kit, erbeta ELISA Kit, Esr2 ELISA Kit, ERBeta2 ELISA Kit, estrogen receptor 2 ELISA Kit, estrogen receptor 2b ELISA Kit, estrogen receptor beta 2 ELISA Kit, estrogen receptor 2 (beta) ELISA Kit, estrogen receptor 2a ELISA Kit, estrogen receptor beta ELISA Kit, ESR2 ELISA Kit, esr2b ELISA Kit, erb2 ELISA Kit, esr2 ELISA Kit, Esr2 ELISA Kit, esr2a ELISA Kit, erbeta ELISA Kit
- 背景
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Gene Name: Estrogen Receptor Beta
Gene Aliases: ER-B, ESRB, ESTRB, NR3A2, NR3-A2, ESR2, Estrogen Receptor 2, Nuclear Receptor Subfamily 3,Group A,Member 2
- 基因ID
- 2100
- UniProt
- Q92731
- 途径
- Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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