EpCAM ELISA 试剂盒
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- 抗原 See all EpCAM (EPCAM) ELISA试剂盒
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.25 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.25 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Epithelial Cell Adhesion Molecule (EPCAM). No significant cross-reactivity or interference between Epithelial Cell Adhesion Molecule (EPCAM) and analogues was observed.
- 灵敏度
- 12.6 pg/mL
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Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit
EPCAM 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epithelial Cell Adhesion Molecule (EPCAM) ELISA KitEPCAM 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Epithelial Cell Adhesion Molecule (EPCAM) ELISA KitEPCAM 适用: 人 AA 24-265 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Epithelial Cell Adhesion Molecule (EPCAM) ELISA KitEPCAM 适用: 人 Colorimetric Sandwich ELISA 93.7 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epithelial Cell Adhesion Molecule (EPCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epithelial Cell Adhesion Molecule (EPCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epithelial Cell Adhesion Molecule (EPCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epithelial Cell Adhesion Molecule (EPCAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epithelial Cell Adhesion Molecule (EPCAM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epithelial Cell Adhesion Molecule (EPCAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all EpCAM (EPCAM) ELISA试剂盒
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- 别名
- Epithelial Cell Adhesion Molecule (EPCAM 产品)
- 别名
- DIAR5 ELISA Kit, EGP-2 ELISA Kit, EGP314 ELISA Kit, EGP40 ELISA Kit, ESA ELISA Kit, HNPCC8 ELISA Kit, KS1/4 ELISA Kit, KSA ELISA Kit, M4S1 ELISA Kit, MIC18 ELISA Kit, MK-1 ELISA Kit, TACSTD1 ELISA Kit, TROP1 ELISA Kit, ECS-1 ELISA Kit, ECS1 ELISA Kit, ESA1 ELISA Kit, M17S1 ELISA Kit, EGP ELISA Kit, Ep-CAM ELISA Kit, sb:cb6 ELISA Kit, tacstd ELISA Kit, wu:fj17g02 ELISA Kit, zgc:110304 ELISA Kit, zgc:77119 ELISA Kit, tacstd1 ELISA Kit, MGC80540 ELISA Kit, EPCAM ELISA Kit, egp ELISA Kit, ksa ELISA Kit, m4s1 ELISA Kit, mk-1 ELISA Kit, cd326 ELISA Kit, egp40 ELISA Kit, mic18 ELISA Kit, trop1 ELISA Kit, ep-cam ELISA Kit, hegp-2 ELISA Kit, co17-1a ELISA Kit, ga733-2 ELISA Kit, CD326 ELISA Kit, Egp314 ELISA Kit, EpCAM1 ELISA Kit, GA733-2 ELISA Kit, Ly74 ELISA Kit, Tacsd1 ELISA Kit, Tacstd1 ELISA Kit, gp40 ELISA Kit, epithelial cell adhesion molecule ELISA Kit, flotillin 2 ELISA Kit, epithelial cell adhesion molecule S homeolog ELISA Kit, EPCAM ELISA Kit, FLOT2 ELISA Kit, epcam ELISA Kit, epcam.S ELISA Kit, Epcam ELISA Kit
- 背景
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Gene Name: Epithelial Cell Adhesion Molecule
Gene Aliases: CD326, TACSTD1, EGP, KSA, M4S1, MIC18, TROP1, HEGP2, ESA, Epithelial glycoprotein, Adenocarcinoma-associated antigen, Tumor Associated Calcium Signal Transducer 1
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