ESM1 ELISA 试剂盒
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- 抗原 See all ESM1 ELISA试剂盒
- ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Endothelial Cell Specific Molecule 1 (ESM1). No significant cross-reactivity or interference between Endothelial Cell Specific Molecule 1 (ESM1) and analogues was observed.
- 灵敏度
- 6.2 pg/mL
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Endothelial Cell-Specific Molecule 1 (ESM1) ELISA Kit
ESM1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 人 AA 20-184 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 小鼠 AA 20-184 Colorimetric Sandwich ELISA 23.4-1500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 小鼠 Colorimetric Sandwich ELISA 23.4 pg/mL - 1500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelial Cell Specific Molecule 1 (ESM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endothelial Cell Specific Molecule 1 (ESM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endothelial Cell Specific Molecule 1 (ESM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endothelial Cell Specific Molecule 1 (ESM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelial Cell Specific Molecule 1 (ESM1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelial Cell Specific Molecule 1 (ESM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all ESM1 ELISA试剂盒
- ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))
- 别名
- Endothelial Cell Specific Molecule 1 (ESM1 产品)
- 别名
- ESM1 ELISA Kit, zgc:153027 ELISA Kit, 0610042H23Rik ELISA Kit, AV004503 ELISA Kit, ESM-1 ELISA Kit, Pg25 ELISA Kit, endocan ELISA Kit, endothelial cell specific molecule 1 ELISA Kit, endothelial cell-specific molecule 1 ELISA Kit, ESM1 ELISA Kit, esm1 ELISA Kit, Esm1 ELISA Kit
- 背景
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Gene Name: Endothelial Cell Specific Molecule 1
Gene Aliases: Endocan
- 基因ID
- 11082
- UniProt
- Q9NQ30
- 途径
- Growth Factor Binding
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