DYNC1H1 ELISA 试剂盒
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- 抗原 See all DYNC1H1 ELISA试剂盒
- DYNC1H1 (Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1). No significant cross-reactivity or interference between Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) and analogues was observed.
- 灵敏度
- 0.113 ng/mL
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Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) ELISA Kit
DYNC1H1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all DYNC1H1 ELISA试剂盒
- DYNC1H1 (Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1))
- 别名
- Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1 产品)
- 别名
- fk70a07 ELISA Kit, wu:fk70a07 ELISA Kit, p22 ELISA Kit, dhc1 ELISA Kit, dncl ELISA Kit, dyhc ELISA Kit, hl-3 ELISA Kit, dhc1a ELISA Kit, dnch1 ELISA Kit, dnecl ELISA Kit, dnchc1 ELISA Kit, Dnch1 ELISA Kit, Dnchc1 ELISA Kit, 9930018I23Rik ELISA Kit, AI894280 ELISA Kit, DHC1 ELISA Kit, DHC1a ELISA Kit, DNCL ELISA Kit, Dnec1 ELISA Kit, Dnecl ELISA Kit, Loa ELISA Kit, MAP1C ELISA Kit, P22 ELISA Kit, Swl ELISA Kit, mKIAA0325 ELISA Kit, DNCH1 ELISA Kit, DNECL ELISA Kit, DYHC ELISA Kit, HL-3 ELISA Kit, SMALED1 ELISA Kit, dynein, cytoplasmic 1, heavy chain 1 ELISA Kit, dynein cytoplasmic 1 heavy chain 1 ELISA Kit, dync1h1 ELISA Kit, DYNC1H1 ELISA Kit, Dync1h1 ELISA Kit
- 背景
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Gene Name: Dynein, Cytoplasmic 1, Heavy Chain 1
Gene Aliases: DHC1, DHC1a, DNCH1, DNCL, DNECL, DYHC, Dnchc1, HL-3, p22, Dynein heavy chain, cytosolic, Cytoplasmic dynein heavy chain 1, Cytoplasmic dynein 1 heavy chain 1
- 基因ID
- 1778
- UniProt
- Q14204
- 途径
- M Phase, Ribonucleoprotein Complex Subunit Organization
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