beta Defensin 1 ELISA 试剂盒
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Quick Overview for beta Defensin 1 ELISA 试剂盒 (ABIN5653576)
抗原
See all beta Defensin 1 (DEFB1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- Bovine Defensin Beta 1 (DEFb1) ELISA Kit for detecting DEFb1 in serum, plasma, tissue homogenates
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Defensin Beta 1 (DEFb1). No significant cross-reactivity or interference between Defensin Beta 1 (DEFb1) and analogues was observed.
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灵敏度
- 0.055 ng/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Defensin, beta 1 (DEFB1) ELISA Kit
DEFB1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Defensin, beta 1 (DEFB1) ELISA KitDEFB1 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Defensin, beta 1 (DEFB1) ELISA KitDEFB1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Defensin, beta 1 (DEFB1) ELISA KitDEFB1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Defensin, beta 1 (DEFB1) ELISA KitDEFB1 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Defensin, beta 1 (DEFB1) ELISA KitDEFB1 适用: 山羊 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Defensin, beta 1 (DEFB1) ELISA KitDEFB1 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Defensin, beta 1 (DEFB1) ELISA KitDEFB1 适用: 大鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Defensin, beta 1 (DEFB1) ELISA KitDEFB1 适用: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Defensin, beta 1 (DEFB1) ELISA KitDEFB1 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 1 (DEFb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 1 (DEFb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 1 (DEFb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 1 (DEFb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Beta 1 (DEFb1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Beta 1 (DEFb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 12 months
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- beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))
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别名
- Defensin Beta 1
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背景
- Gene Aliases: B-DF1, DEFB1, BD1, DEF-B1, DEFB101, HBD1
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UniProt
- P46159
抗原 See all beta Defensin 1 (DEFB1) ELISA试剂盒
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