COX IV ELISA 试剂盒
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- 抗原 See all COX IV (COX4I1) ELISA试剂盒
- COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1). No significant cross-reactivity or interference between Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) and analogues was observed.
- 灵敏度
- 0.058 ng/mL
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Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) ELISA Kit
COX4I1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) ELISA KitCOX4I1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) ELISA KitCOX4I1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) ELISA KitCOX4I1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) ELISA KitCOX4I1 适用: 人 Isoform 1 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all COX IV (COX4I1) ELISA试剂盒
- COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))
- 别名
- Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1 产品)
- 别名
- COX4 ELISA Kit, COX4-1 ELISA Kit, COXIV ELISA Kit, mg:bb02d03 ELISA Kit, zgc:110058 ELISA Kit, GB20012 ELISA Kit, AL024441 ELISA Kit, Cox4 ELISA Kit, Cox4a ELISA Kit, cox4 ELISA Kit, cox4i1 ELISA Kit, coxiv ELISA Kit, BcDNA:AT07685 ELISA Kit, CG10396 ELISA Kit, COX IV ELISA Kit, CX41 ELISA Kit, CX42 ELISA Kit, Dmel\CG10396 ELISA Kit, IV ELISA Kit, cytochrome c oxidase subunit 4I1 ELISA Kit, cytochrome c oxidase subunit IV isoform 1 ELISA Kit, cytochrome c oxidase subunit 4 isoform 1, mitochondrial ELISA Kit, cytochrome c oxidase polypeptide IV ELISA Kit, cytochrome c oxidase subunit IV ELISA Kit, cytochrome c oxidase subunit 4i1 ELISA Kit, cytochrome c oxidase subunit IV isoform 1 S homeolog ELISA Kit, Cytochrome c oxidase subunit 4-like ELISA Kit, COX4I1 ELISA Kit, cox4i1 ELISA Kit, LOC412396 ELISA Kit, Cox4I1 ELISA Kit, LOC780848 ELISA Kit, BMEII0322 ELISA Kit, RSal33209_1582 ELISA Kit, SJAG_00976 ELISA Kit, HMPREF0733_11926 ELISA Kit, Cox4i1 ELISA Kit, cox4i1.S ELISA Kit, COX4L ELISA Kit
- 背景
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Gene Name: Cytochrome C Oxidase Subunit IV Isoform 1
Gene Aliases: COX4, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
- 途径
- Proton Transport
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