Coagulation Factor V ELISA 试剂盒
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- 抗原 See all Coagulation Factor V (F5) ELISA试剂盒
- Coagulation Factor V (F5)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 125.00 pg/mL - 8000 pg/mL
- 最低检测浓度
- 125.00 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Coagulation Factor V (F5). No significant cross-reactivity or interference between Coagulation Factor V (F5) and analogues was observed.
- 灵敏度
- 55 pg/mL
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Coagulation Factor V (F5) ELISA Kit
F5 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma
Coagulation Factor V (F5) ELISA KitF5 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma
Coagulation Factor V (F5) ELISA KitF5 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Coagulation Factor V (F5) ELISA KitF5 适用: 人 Colorimetric Sandwich ELISA 15.625 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor V (F5) ELISA KitF5 适用: 大鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor V (F5) ELISA KitF5 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor V (F5) ELISA KitF5 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma
Coagulation Factor V (F5) ELISA KitF5 适用: 小鼠 Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum
Coagulation Factor V (F5) ELISA KitF5 适用: 人 Colorimetric Competition ELISA 50-1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Coagulation Factor V (F5) ELISA KitF5 适用: 猴 Colorimetric Competition ELISA 50-1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor V (F5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor V (F5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor V (F5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor V (F5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor V (F5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor V (F5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Coagulation Factor V (F5) ELISA试剂盒
- Coagulation Factor V (F5)
- 别名
- Coagulation Factor V (F5 产品)
- 别名
- AI173222 ELISA Kit, Cf-5 ELISA Kit, Cf5 ELISA Kit, FVL ELISA Kit, PCCF ELISA Kit, RPRGL1 ELISA Kit, THPH2 ELISA Kit, Ac2-120 ELISA Kit, coagulation factor V ELISA Kit, f5 ELISA Kit, F5 ELISA Kit
- 背景
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Gene Name: Coagulation Factor V
Gene Aliases: FV, FVL, PCCF, Proaccelerin, Labile Factor, Factor V Leiden, Accelerator Globulin, Activated protein C cofactor, Proaccelerin, labile factor
- 基因ID
- 2153
- UniProt
- P12259
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