CHRNA1 CLIA Kit
-
- 抗原 See all CHRNA1 CLIA Kits
- CHRNA1 (Acetylcholine Receptor Subunit alpha (CHRNA1))
-
适用
- 人
- 检测方法
- Chemiluminescent
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.781 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.781 ng/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1). No significant cross-reactivity or interference between Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) and analogues was observed.
- 灵敏度
- 0.03 ng/mL
- Top Product
- Discover our top product CHRNA1 ELISA Kit
-
Acetylcholine Receptor Subunit alpha (CHRNA1) ELISA Kit
CHRNA1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Acetylcholine Receptor Subunit alpha (CHRNA1) ELISA KitCHRNA1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
-
- 说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 - 3 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) level in the sample or standard.,
- 实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
-
- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all CHRNA1 CLIA Kits
- CHRNA1 (Acetylcholine Receptor Subunit alpha (CHRNA1))
- 别名
- Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNA1 产品)
- 别名
- CHRNA1 CLIA Kit, ACHRA CLIA Kit, ACHRD CLIA Kit, CHRNA CLIA Kit, CMS2A CLIA Kit, FCCMS CLIA Kit, SCCMS CLIA Kit, NACHRA1 CLIA Kit, AI385656 CLIA Kit, AI608266 CLIA Kit, Achr-1 CLIA Kit, Acra CLIA Kit, cholinergic receptor nicotinic alpha 1 subunit CLIA Kit, cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) CLIA Kit, CHRNA1 CLIA Kit, Chrna1 CLIA Kit
- 背景
-
Gene Name: Cholinergic Receptor, Nicotinic, Alpha 1
Gene Aliases: CHR-NA1, N-AChRA1, NAChRA1, ACHRA, ACHRD, CMS2A, FCCMS, SCCMS, Acetylcholine receptor subunit alpha, N-AChR-A1, Neuronal Acetylcholine Receptor Alpha 1,Muscle
- 基因ID
- 1134
- UniProt
- P02708
- 途径
- Skeletal Muscle Fiber Development
-
