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CXCR4 ELISA 试剂盒

CXCR4 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
产品编号 ABIN5652851
发货至: 中国
  • 抗原 See all CXCR4 ELISA试剂盒
    CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    78 pg/mL - 5000 pg/mL
    最低检测浓度
    78 pg/mL
    应用范围
    ELISA
    样品类型
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Chemokine C-X-C-Motif Receptor 4 (CXCR4). No significant cross-reactivity or interference between Chemokine C-X-C-Motif Receptor 4 (CXCR4) and analogues was observed.
    灵敏度
    31 pg/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X-C-Motif Receptor 4 (CXCR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-X-C-Motif Receptor 4 (CXCR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X-C-Motif Receptor 4 (CXCR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-X-C-Motif Receptor 4 (CXCR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-X-C-Motif Receptor 4 (CXCR4) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-X-C-Motif Receptor 4 (CXCR4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all CXCR4 ELISA试剂盒
    CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))
    别名
    Chemokine C-X-C-Motif Receptor 4 (CXCR4 产品)
    别名
    CD184 ELISA Kit, D2S201E ELISA Kit, FB22 ELISA Kit, HM89 ELISA Kit, HSY3RR ELISA Kit, LAP3 ELISA Kit, LCR1 ELISA Kit, LESTR ELISA Kit, NPY3R ELISA Kit, NPYR ELISA Kit, NPYRL ELISA Kit, NPYY3R ELISA Kit, WHIM ELISA Kit, Cmkar4 ELISA Kit, PB-CKR ELISA Kit, PBSF/SDF-1 ELISA Kit, Sdf1r ELISA Kit, CXC-R4-B ELISA Kit, CXCR-4-B ELISA Kit, cd184 ELISA Kit, cxcr4 ELISA Kit, fb22 ELISA Kit, hm89 ELISA Kit, hsy3rr ELISA Kit, lap3 ELISA Kit, lcr1 ELISA Kit, lestr ELISA Kit, npy3r ELISA Kit, npyr ELISA Kit, npyrl ELISA Kit, npyy3r ELISA Kit, xcxcr4 ELISA Kit, CXC-R4 ELISA Kit, CXCR-4 ELISA Kit, d2s201e ELISA Kit, whim ELISA Kit, CXC-R4-A ELISA Kit, CXCR-4-A ELISA Kit, xCXCR4 ELISA Kit, CXCR4 ELISA Kit, cb403 ELISA Kit, zgc:109863 ELISA Kit, cb824 ELISA Kit, C-X-C motif chemokine receptor 4 ELISA Kit, chemokine (C-X-C motif) receptor 4 ELISA Kit, C-X-C motif chemokine receptor 4 S homeolog ELISA Kit, C-X-C chemokine receptor type 4 ELISA Kit, C-X-C motif chemokine receptor 4 L homeolog ELISA Kit, chemokine (C-X-C motif), receptor 4b ELISA Kit, chemokine (C-X-C motif) receptor 4a ELISA Kit, CXCR4 ELISA Kit, Cxcr4 ELISA Kit, cxcr4.S ELISA Kit, cxcr4 ELISA Kit, LOC100049444 ELISA Kit, cxcr4.L ELISA Kit, cxcr4b ELISA Kit, cxcr4a ELISA Kit
    背景

    Gene Name: Chemokine C-X-C-Motif Receptor 4

    Gene Aliases: CD184, Fusin, NPYR, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYRL, WHIM, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide-associated protein 3

    途径
    Regulation of Cell Size, CXCR4-mediated Signaling Events
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