Calretinin ELISA 试剂盒
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- 抗原 See all Calretinin (CALB2) ELISA试剂盒
- Calretinin (CALB2) (Calbindin 2 (CALB2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Calretinin (CR). No significant cross-reactivity or interference between Calretinin (CR) and analogues was observed.
- 灵敏度
- 0.059 ng/mL
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Calbindin 2 (CALB2) ELISA Kit
CALB2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Calbindin 2 (CALB2) ELISA KitCALB2 适用: 大鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Calbindin 2 (CALB2) ELISA KitCALB2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Calbindin 2 (CALB2) ELISA KitCALB2 适用: 犬 Colorimetric Sandwich ELISA 50-1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calretinin (CR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calretinin (CR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calretinin (CR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calretinin (CR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calretinin (CR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calretinin (CR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Calretinin (CALB2) ELISA试剂盒
- Calretinin (CALB2) (Calbindin 2 (CALB2))
- 别名
- Calretinin (CALB2 产品)
- 别名
- CAB29 ELISA Kit, CAL2 ELISA Kit, CR ELISA Kit, calb2l ELISA Kit, wu:fq18e08 ELISA Kit, zgc:73115 ELISA Kit, calb2 ELISA Kit, wu:fq17g09 ELISA Kit, zgc:73099 ELISA Kit, calbindin 2 ELISA Kit, calbindin 2a ELISA Kit, calbindin 2b ELISA Kit, CALB2 ELISA Kit, Calb2 ELISA Kit, calb2a ELISA Kit, calb2b ELISA Kit
- 背景
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Gene Name: Calretinin
Gene Aliases: CALB2, CAL2, CAB29, Calbindin 2,29 kDa
- 基因ID
- 794
- UniProt
- P22676
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