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Calreticulin ELISA 试剂盒

CALR 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
产品编号 ABIN5652527
发货至: 中国
  • 抗原 See all Calreticulin (CALR) ELISA试剂盒
    Calreticulin (CALR)
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156 ng/mL - 10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Calreticulin (CRT). No significant cross-reactivity or interference between Calreticulin (CRT) and analogues was observed.
    灵敏度
    0.067 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calreticulin (CRT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calreticulin (CRT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calreticulin (CRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calreticulin (CRT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calreticulin (CRT) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calreticulin (CRT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all Calreticulin (CALR) ELISA试剂盒
    Calreticulin (CALR)
    别名
    Calreticulin (CALR 产品)
    别名
    CRT ELISA Kit, RO ELISA Kit, SSA ELISA Kit, cC1qR ELISA Kit, crc ELISA Kit, crt ELISA Kit, Calregulin ELISA Kit, CG9429 ELISA Kit, Clr ELISA Kit, Dmel\\CG9429 ELISA Kit, anon-EST:Liang-2.11 ELISA Kit, anon-EST:Liang-2.17 ELISA Kit, anon-EST:fe3D7 ELISA Kit, clone 2.11 ELISA Kit, clone 2.17 ELISA Kit, eth ELISA Kit, potp ELISA Kit, cc1qr ELISA Kit, ssa ELISA Kit, NV19007 ELISA Kit, CRP55 ELISA Kit, ERp60 ELISA Kit, HACBP ELISA Kit, CHOT1 ELISA Kit, CHT1 ELISA Kit, CT1 ELISA Kit, AA589632 ELISA Kit, CRTR ELISA Kit, Creat ELISA Kit, CCDS1 ELISA Kit, calrl2 ELISA Kit, fc66d10 ELISA Kit, wu:fc66d10 ELISA Kit, wu:fj50c03 ELISA Kit, zgc:66153 ELISA Kit, calreticulin ELISA Kit, calreticulin L homeolog ELISA Kit, Calreticulin ELISA Kit, putative calreticulin ELISA Kit, solute carrier family 6 member 8 ELISA Kit, solute carrier family 6 (neurotransmitter transporter, creatine), member 8 ELISA Kit, CALR ELISA Kit, calr.L ELISA Kit, Crt ELISA Kit, crt ELISA Kit, CRT ELISA Kit, Calr ELISA Kit, calr ELISA Kit, LMJF_31_2600 ELISA Kit, Crc ELISA Kit, Slc6a8 ELISA Kit, SLC6A8 ELISA Kit, LOC8269812 ELISA Kit
    背景

    Gene Name: Calreticulin

    Gene Aliases: CALR, RO, SSA, CC1qR, ERp60, HACBP, grp60, CRTC, CRP55, Calregulin, Sicca Syndrome Antigen A, Autoantigen Ro, Endoplasmic reticulum resident protein 60

    基因ID
    811
    UniProt
    P27797
    途径
    Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response
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