CGRP CLIA Kit
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- 抗原 See all CGRP (CALCA) CLIA Kits
- CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))
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适用
- 小鼠
- 检测方法
- Chemiluminescent
- 实验类型
- Competition ELISA
- 检测范围
- 7.81 pg/mL - 2000 pg/mL
- 最低检测浓度
- 7.81 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Calcitonin Gene Related Peptide (CGRP). No significant cross-reactivity or interference between Calcitonin Gene Related Peptide (CGRP) and analogues was observed.
- 灵敏度
- 2.77 pg/mL
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- Discover our top product CALCA ELISA Kit
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Calcitonin-Related Polypeptide alpha (CALCA) ELISA Kit
CALCA 适用: 人 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 小鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 小鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Calcitonin-Related Polypeptide alpha (CALCA) ELISA KitCALCA 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Calcitonin Gene Related Peptide (CGRP). A competitive inhibition reaction is launched between biotin labeled Calcitonin Gene Related Peptide (CGRP) and unlabeled Calcitonin Gene Related Peptide (CGRP) (Standards or samples) with the pre-coated antibody specific to Calcitonin Gene Related Peptide (CGRP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Calcitonin Gene Related Peptide (CGRP) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Calcitonin Gene Related Peptide (CGRP) level in the sample or standard.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all CGRP (CALCA) CLIA Kits
- CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))
- 别名
- Calcitonin Gene Related Peptide (CALCA 产品)
- 别名
- CALC1 CLIA Kit, CGRP CLIA Kit, CGRP-I CLIA Kit, CGRP1 CLIA Kit, CT CLIA Kit, KC CLIA Kit, CALC CLIA Kit, CALCA CLIA Kit, CALC3 CLIA Kit, ci-ct CLIA Kit, calca CLIA Kit, CA CLIA Kit, CGRP-1 CLIA Kit, Calc CLIA Kit, Calc1 CLIA Kit, Cgrp CLIA Kit, Ct CLIA Kit, Ctn CLIA Kit, calcitonin CLIA Kit, CAL6 CLIA Kit, Cal1 CLIA Kit, RATCAL6 CLIA Kit, CGRPI CLIA Kit, calc CLIA Kit, cgrp CLIA Kit, zgc:92886 CLIA Kit, calcitonin related polypeptide alpha CLIA Kit, calcitonin related polypeptide beta CLIA Kit, calcitonin-related polypeptide alpha CLIA Kit, calcitonin CLIA Kit, calcitonin/calcitonin-related polypeptide, alpha CLIA Kit, calcitonin-related polypeptide beta CLIA Kit, calcitonin gene-related peptide 2 CLIA Kit, CALCA CLIA Kit, CALCB CLIA Kit, ci-ct CLIA Kit, calca CLIA Kit, Calca CLIA Kit, LOC100008771 CLIA Kit
- 背景
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Gene Name: Calcitonin Gene Related Peptide
Gene Aliases: CALCB, CGRP2, CALC2, CGRP-II, Calcitonin Gene Related Peptide 2, Calcitonin 2, Calcitonin Related Polypeptide Beta
- UniProt
- Q99MP3
- 途径
- Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour
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