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CRP ELISA 试剂盒

CRP 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
产品编号 ABIN5652371
发货至: 中国
  • 抗原 See all CRP ELISA试剂盒
    CRP (C-Reactive Protein (CRP))
    适用
    • 8
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156 ng/mL - 10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of C Reactive Protein (CRP). No significant cross-reactivity or interference between C Reactive Protein (CRP) and analogues was observed.
    灵敏度
    0.065 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C Reactive Protein (CRP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C Reactive Protein (CRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C Reactive Protein (CRP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C Reactive Protein (CRP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C Reactive Protein (CRP) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C Reactive Protein (CRP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all CRP ELISA试剂盒
    CRP (C-Reactive Protein (CRP))
    别名
    C Reactive Protein (CRP 产品)
    别名
    PTX1 ELISA Kit, crp ELISA Kit, AI255847 ELISA Kit, Aa1249 ELISA Kit, Ab1-341 ELISA Kit, Ab2-196 ELISA Kit, Ac1-114 ELISA Kit, Ac1262 ELISA Kit, Ac2-069 ELISA Kit, Ba2-693 ELISA Kit, APCS ELISA Kit, 0610010I23Rik ELISA Kit, AW743261 ELISA Kit, C77570 ELISA Kit, CRP2 ELISA Kit, CRP4 ELISA Kit, Crp ELISA Kit, ESP1 ELISA Kit, Hlp ELISA Kit, CRP5.1 ELISA Kit, zgc:152809 ELISA Kit, C-reactive protein ELISA Kit, C-reactive protein, pentraxin-related ELISA Kit, c-reactive protein, pentraxin-related ELISA Kit, cysteine rich protein 2 ELISA Kit, CRP ELISA Kit, crp ELISA Kit, Crp ELISA Kit, Crip2 ELISA Kit, LOC776376 ELISA Kit
    背景

    Gene Name: C Reactive Protein

    Gene Aliases: C-RP, PTX1, Pentraxin-Related, Pentraxin 1

    基因ID
    12944
    UniProt
    P14847
    途径
    Carbohydrate Homeostasis
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