CD86 ELISA 试剂盒
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- 抗原 See all CD86 ELISA试剂盒
- CD86
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of B-Lymphocyte Activation Antigen B7-2 (LAB7-2). No significant cross-reactivity or interference between B-Lymphocyte Activation Antigen B7-2 (LAB7-2) and analogues was observed.
- 灵敏度
- 0.274 ng/mL
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CD86 (CD86) ELISA Kit
CD86 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD86 (CD86) ELISA KitCD86 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
CD86 (CD86) ELISA KitCD86 适用: Cow Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
CD86 (CD86) ELISA KitCD86 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
CD86 (CD86) ELISA KitCD86 适用: 大鼠 AA 29-250 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD86 (CD86) ELISA KitCD86 适用: 小鼠 AA 26-245 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Activation Antigen B7-2 (LAB7-2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Lymphocyte Activation Antigen B7-2 (LAB7-2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Lymphocyte Activation Antigen B7-2 (LAB7-2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Lymphocyte Activation Antigen B7-2 (LAB7-2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Lymphocyte Activation Antigen B7-2 (LAB7-2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Lymphocyte Activation Antigen B7-2 (LAB7-2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all CD86 ELISA试剂盒
- CD86
- 别名
- B-Lymphocyte Activation Antigen B7-2 (CD86 产品)
- 别名
- B7-2 ELISA Kit, B7.2 ELISA Kit, B70 ELISA Kit, CD28LG2 ELISA Kit, LAB72 ELISA Kit, B7 ELISA Kit, CLS1 ELISA Kit, Cd28l2 ELISA Kit, ETC-1 ELISA Kit, Ly-58 ELISA Kit, Ly58 ELISA Kit, MB7 ELISA Kit, MB7-2 ELISA Kit, TS/A-2 ELISA Kit, CD86 molecule ELISA Kit, CD86 antigen ELISA Kit, integrin subunit alpha 2b ELISA Kit, CD86 ELISA Kit, Cd86 ELISA Kit, Itga2b ELISA Kit
- 背景
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Gene Name: B-Lymphocyte Activation Antigen B7-2
Gene Aliases: CD86, B7-2, B70, FUN-1, CD28LG2, CD28 Antigen Ligand 2,B7-2 Antigen, CTLA-4 counter-receptor B7.2
- 基因ID
- 942
- UniProt
- P42081
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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