ATP1A1 ELISA 试剂盒
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Quick Overview for ATP1A1 ELISA 试剂盒 (ABIN5652094)
抗原
See all ATP1A1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- Human/Mouse/Rat/Pig ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) ELISA Kit for detecting ATP1a1 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). No significant cross-reactivity or interference between ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) and analogues was observed.
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灵敏度
- 0.062 ng/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Sodium Potassium ATPase, alpha1 (ATP1A1) ELISA Kit
ATP1A1 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Sodium Potassium ATPase, alpha1 (ATP1A1) ELISA KitATP1A1 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Sodium Potassium ATPase, alpha1 (ATP1A1) ELISA KitATP1A1 适用: 人, 大鼠, 小鼠, Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Sodium Potassium ATPase, alpha1 (ATP1A1) ELISA KitATP1A1 适用: 人 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 12 months
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- ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))
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别名
- ATPase, Na+/K+ Transporting Alpha 1 Polypeptide
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背景
- Gene Aliases: Sodium pump subunit alpha-1, Na (+) /K (+) ATPase alpha-1 subunit, Sodium/potassium-transporting ATPase subunit alpha-1
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基因ID
- 476
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UniProt
- P05023
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途径
- Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process
抗原 See all ATP1A1 ELISA试剂盒
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