APOE ELISA 试剂盒
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- 抗原 See all APOE ELISA试剂盒
- APOE (Apolipoprotein E (APOE))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 6.25 ng/mL - 400 ng/mL
- 最低检测浓度
- 6.25 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Apolipoprotein E (APOE). No significant cross-reactivity or interference between Apolipoprotein E (APOE) and analogues was observed.
- 灵敏度
- 2.55 ng/mL
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Apolipoprotein E (APOE) ELISA Kit
APOE 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 小鼠 Colorimetric Competition ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 大鼠 Colorimetric Competition ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 人 Colorimetric Sandwich ELISA 4.7 ng/mL - 300 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: Pig Colorimetric Competition ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 人 Colorimetric Competition ELISA 15.62 ng/mL - 1000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 犬 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 猴 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein E (APOE) ELISA KitAPOE 适用: 猴 Colorimetric Competition ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Saliva, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein E (APOE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein E (APOE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein E (APOE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein E (APOE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein E (APOE) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein E (APOE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all APOE ELISA试剂盒
- APOE (Apolipoprotein E (APOE))
- 别名
- Apolipoprotein E (APOE 产品)
- 别名
- ad2 ELISA Kit, apoprotein ELISA Kit, im:7036787 ELISA Kit, wu:fb69a05 ELISA Kit, zgc:110064 ELISA Kit, apoe ELISA Kit, AI255918 ELISA Kit, AD2 ELISA Kit, LDLCQ5 ELISA Kit, LPG ELISA Kit, APOEA ELISA Kit, Apo-E ELISA Kit, apolipoprotein E ELISA Kit, apolipoprotein Ea ELISA Kit, apoe ELISA Kit, apoea ELISA Kit, Apoe ELISA Kit, APOE ELISA Kit
- 背景
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Gene Name: Apolipoprotein E
Gene Aliases: Apo-E, AD2, Apoprotein, Alzheimer Disease 2(E4-Associated,Late Onset
- 基因ID
- 25728
- UniProt
- P02650
- 途径
- Regulation of Cell Size, Lipid Metabolism
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