AMH CLIA Kit
Our Local Distributor
北京 101111
Quick Overview for AMH CLIA Kit (ABIN5651851)
抗原
See all AMH CLIA Kits适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 23.44 pg/mL
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Anti-Mullerian Hormone (AMH). No significant cross-reactivity or interference between Anti-Mullerian Hormone (AMH) and analogues was observed.
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灵敏度
- 8.65 pg/mL
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Anti-Mullerian Hormone (AMH) ELISA Kit
AMH 适用: 人 Colorimetric Competition ELISA 937.5 pg/mL - 15000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: 大鼠 Colorimetric Competition ELISA 61.73 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: Pig Colorimetric Competition ELISA 1.88 ng/mL - 30 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: 小鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Plasma, Serum
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: Cow Colorimetric Competition ELISA 61.73 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: 人 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: 人 Colorimetric Competition ELISA 937.5 pg/mL - 15000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: 小鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Plasma, Serum
Anti-Mullerian Hormone (AMH) ELISA KitAMH 适用: 小鸡 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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实验时间
- 2 h
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板类型
- Pre-coated
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实验流程
- The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Anti-Mullerian Hormone (AMH). A competitive inhibition reaction is launched between biotin labeled Anti-Mullerian Hormone (AMH) and unlabeled Anti-Mullerian Hormone (AMH) (Standards or samples) with the pre-coated antibody specific to Anti-Mullerian Hormone (AMH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Anti-Mullerian Hormone (AMH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Anti-Mullerian Hormone (AMH) level in the sample or standard.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 4-8 months
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- AMH (Anti-Mullerian Hormone (AMH))
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别名
- Anti-Mullerian Hormone
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背景
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Gene Name: Anti-Mullerian Hormone
Gene Aliases: MIF, MIH, MIS, Müllerian Inhibiting Factor, Müllerian Inhibiting Hormone, Müllerian Inhibiting Substance
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基因ID
- 268
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UniProt
- P03971
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途径
- Negative Regulation of Hormone Secretion
抗原 See all AMH CLIA Kits
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