ANGPTL4 ELISA 试剂盒
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- 抗原 See all ANGPTL4 ELISA试剂盒
- ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56 ng/mL - 100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Angiopoietin Like Protein 4 (ANGPTL4). No significant cross-reactivity or interference between Angiopoietin Like Protein 4 (ANGPTL4) and analogues was observed.
- 灵敏度
- 0.59 ng/mL
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Angiopoietin-Like 4 (ANGPTL4) ELISA Kit
ANGPTL4 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 小鼠 Colorimetric Competition ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 人 AA 165-406 Colorimetric Sandwich ELISA 625-40000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 山羊 Colorimetric Competition ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 人 Colorimetric Sandwich ELISA 625 pg/mL - 40000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Angiopoietin-Like 4 (ANGPTL4) ELISA KitANGPTL4 适用: 大鼠 Colorimetric Sandwich ELISA 7.8-500 pg/mL Bronchoalveolar Lavage, Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 4 (ANGPTL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin Like Protein 4 (ANGPTL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 4 (ANGPTL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiopoietin Like Protein 4 (ANGPTL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin Like Protein 4 (ANGPTL4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin Like Protein 4 (ANGPTL4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all ANGPTL4 ELISA试剂盒
- ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))
- 别名
- Angiopoietin Like Protein 4 (ANGPTL4 产品)
- 别名
- ANGPTL2 ELISA Kit, ARP4 ELISA Kit, FIAF ELISA Kit, HFARP ELISA Kit, NL2 ELISA Kit, PGAR ELISA Kit, pp1158 ELISA Kit, Arp4 ELISA Kit, Bk89 ELISA Kit, Fiaf ELISA Kit, Hfarp ELISA Kit, Ng27 ELISA Kit, Pgar ELISA Kit, Pgarg ELISA Kit, Pp1158 ELISA Kit, ANGPTL4 ELISA Kit, fiaf ELISA Kit, im:7144703 ELISA Kit, si:rp71-39b20.7 ELISA Kit, angiopoietin like 4 ELISA Kit, angiopoietin-like 4 ELISA Kit, ANGPTL4 ELISA Kit, Angptl4 ELISA Kit, angptl4 ELISA Kit
- 背景
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Gene Name: Angiopoietin Like Protein 4
Gene Aliases: ARP4, FIAF, HFARP, NL2, PGAR, Pp1158, Fasting-Induced Adipose Factor, Hepatic Angiopoietin-Related Protein, Hepatic Fibrinogen/Angiopoietin-Related Protein
- 基因ID
- 362850
- UniProt
- Q6TMA8
- 途径
- Regulation of Lipid Metabolism by PPARalpha
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