Androsterone (ADT) ELISA 试剂盒
The Various Species Androsterone (ADT) ELISA Kit (ABIN5651719) is a Colorimetric ELISA Kit designed to quantify Various Species Androsterone (ADT).
产品编号 ABIN5651719
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Androsterone (ADT) ELISA 试剂盒 (ABIN5651719)
抗原
Androsterone (ADT)
适用
Various Species
检测方法
Colorimetric
实验类型
Competition ELISA
检测范围
352 pg/mL - 90000 pg/mL
应用范围
ELISA
样品类型
Plasma, Serum
-
-
最低检测浓度
- 352 pg/mL
-
原理
- Androsterone (ADT) ELISA Kit for detecting ADT in serum, plasma
-
Analytical Method
- Quantitative
-
特异性
- This assay has high sensitivity and excellent specificity for detection of Androsterone (ADT). No significant cross-reactivity or interference between Androsterone (ADT) and analogues was observed.
-
灵敏度
- 144 pg/mL
-
组件
-
- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
-
试剂未包括
-
- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
-
-
Androsterone (ADT) ELISA Kit
适用: Various Species Colorimetric Competition ELISA 352 pg/mL - 90000 pg/mL
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
-
样本量
- 50 μL
-
实验时间
- 2 h
-
板类型
- Pre-coated
-
实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Androsterone (ADT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Androsterone (ADT) and unlabeled Androsterone (ADT) (Standards or samples) with the pre-coated antibody specific to Androsterone (ADT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Androsterone (ADT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Androsterone (ADT) in the sample.
-
实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Androsterone (ADT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Androsterone (ADT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
-
-
-
注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
-
有效期
- 12 months
-
-
- Androsterone (ADT)
-
别名
- Androsterone
-
物质类
- Hormone
-
背景
- Gene Aliases: 17-Ketosteroid, 17-KS, 3a-Hydroxy-17-Androstanone
抗原
-
You are here:
