alpha 2 Macroglobulin CLIA Kit
Our Local Distributor
北京 101111
Quick Overview for alpha 2 Macroglobulin CLIA Kit (ABIN5651629)
抗原
See all alpha 2 Macroglobulin (A2M) CLIA Kits适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 0.39 ng/mL
-
Analytical Method
- Quantitative
-
特异性
- This assay has high sensitivity and excellent specificity for detection of Alpha-2-Macroglobulin (a2M). No significant cross-reactivity or interference between Alpha-2-Macroglobulin (a2M) and analogues was observed.
-
灵敏度
- 0.05 ng/mL
-
-
alpha-2-Macroglobulin (A2M) ELISA Kit
A2M 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitHigh Sensitivity A2M 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 小鼠 Colorimetric Competition ELISA 3.7 ng/mL - 300 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Competition ELISA 1.25 μg/mL - 20 μg/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: Cow Colorimetric Competition ELISA 0.312 μg/mL - 20 μg/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 大鼠 Colorimetric Competition ELISA 123.5 ng/mL - 10000 ng/mL Plasma, Serum
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Sandwich ELISA 78.125 ng/mL - 5000 ng/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 大鼠 Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: Cow Colorimetric Sandwich ELISA 0.156 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
-
-
说明
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
-
实验时间
- 2 - 3 h
-
板类型
- Pre-coated
-
实验流程
- The microplate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Macroglobulin (a2M). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin (a2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Alpha-2-Macroglobulin (a2M) level in the sample or standard.,
-
实验精密度
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Macroglobulin (a2M) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Macroglobulin (a2M) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
-
-
-
注意事项
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
-
有效期
- 4-8 months
-
-
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
-
别名
- Alpha-2-Macroglobulin
-
背景
-
Gene Name: Alpha-2-Macroglobulin
Gene Aliases: A2MG, A2 mg, CPAMD5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
-
基因ID
- 2
-
UniProt
- P01023
-
途径
- Lipid Metabolism
抗原 See all alpha 2 Macroglobulin (A2M) CLIA Kits
-
