alpha 2 Macroglobulin ELISA 试剂盒
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- 抗原 See all alpha 2 Macroglobulin (A2M) ELISA试剂盒
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 3.7 ng/mL - 300 ng/mL
- 最低检测浓度
- 3.7 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Urine
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Alpha-2-Macroglobulin (a2M). No significant cross-reactivity or interference between Alpha-2-Macroglobulin (a2M) and analogues was observed.
- 灵敏度
- 1.51 ng/mL
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alpha-2-Macroglobulin (A2M) ELISA Kit
A2M 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 小鼠 Colorimetric Competition ELISA 3.7 ng/mL - 300 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitHigh Sensitivity A2M 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Competition ELISA 1.25 μg/mL - 20 μg/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Cell Culture Supernatant, Plasma, Serum
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Sandwich ELISA 78.125 ng/mL - 5000 ng/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 大鼠 Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: Cow Colorimetric Sandwich ELISA 0.156 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 兔 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Alpha-2-Macroglobulin (a2M) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Alpha-2-Macroglobulin (a2M) and unlabeled Alpha-2-Macroglobulin (a2M) (Standards or samples) with the pre-coated antibody specific to Alpha-2-Macroglobulin (a2M). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Alpha-2-Macroglobulin (a2M) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Alpha-2-Macroglobulin (a2M) in the sample.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Macroglobulin (a2M) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Macroglobulin (a2M) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
-
- 抗原 See all alpha 2 Macroglobulin (A2M) ELISA试剂盒
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
- 别名
- Alpha-2-Macroglobulin (A2M 产品)
- 别名
- A2MD ELISA Kit, CPAMD5 ELISA Kit, FWP007 ELISA Kit, S863-7 ELISA Kit, A2MAC1 ELISA Kit, A2m1 ELISA Kit, A2maa ELISA Kit, Mam ELISA Kit, A2mp ELISA Kit, A2M ELISA Kit, LOC733429 ELISA Kit, endod ELISA Kit, cpamd5 ELISA Kit, fwp007 ELISA Kit, s863-7 ELISA Kit, a2mb ELISA Kit, endodermin ELISA Kit, Alpha-2-M ELISA Kit, alpha-2-macroglobulin ELISA Kit, pregnancy-zone protein L homeolog ELISA Kit, alpha-2-macroglobulin S homeolog ELISA Kit, pregnancy zone protein ELISA Kit, alpha-2-macroglobulin-like ELISA Kit, A2M ELISA Kit, A2m ELISA Kit, LOC477699 ELISA Kit, pzp.L ELISA Kit, AZL_c00450 ELISA Kit, LOC100349077 ELISA Kit, a2m ELISA Kit, LOC100061656 ELISA Kit, LOC100090399 ELISA Kit, a2m.S ELISA Kit, LOC100469973 ELISA Kit, LOC100595735 ELISA Kit, PZP ELISA Kit, LOC100353095 ELISA Kit, LOC101122940 ELISA Kit, LOC101801552 ELISA Kit, LOC100911545 ELISA Kit
- 背景
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Gene Name: Alpha-2-Macroglobulin
Gene Aliases: A2MG, A2 mg, CPAMD5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
- UniProt
- Q61838
- 途径
- Lipid Metabolism
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