PACAP ELISA 试剂盒
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- 抗原 See all PACAP (ADCYAP1) ELISA试剂盒
- PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 6.17 pg/mL - 500 pg/mL
- 最低检测浓度
- 6.17 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). No significant cross-reactivity or interference between Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) and analogues was observed.
- 灵敏度
- 2.55 pg/mL
- Featured
- Discover our best selling ADCYAP1 ELISA Kit
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- Discover our top product ADCYAP1 ELISA Kit
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Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1) ELISA Kit
ADCYAP1 适用: 人 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1) ELISA KitADCYAP1 适用: 大鼠 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum
Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1) ELISA KitADCYAP1 适用: 大鼠 Colorimetric Competition ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1) ELISA KitADCYAP1 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1) ELISA KitADCYAP1 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1) ELISA KitADCYAP1 适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum
Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1) ELISA KitADCYAP1 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1) ELISA KitADCYAP1 适用: 兔 Colorimetric Competition ELISA 50-1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) and unlabeled Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) (Standards or samples) with the pre-coated antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the sample.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all PACAP (ADCYAP1) ELISA试剂盒
- PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))
- 别名
- Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1 产品)
- 别名
- PACAP ELISA Kit, grf ELISA Kit, Pacap ELISA Kit, adcyap1 ELISA Kit, GRF-PACAP ELISA Kit, zgc:103760 ELISA Kit, xx:af217251 ELISA Kit, pacap ELISA Kit, ADCYAP1 ELISA Kit, PACAP2 ELISA Kit, acap1l ELISA Kit, pacap1b ELISA Kit, wu:fj30e08 ELISA Kit, zgc:101522 ELISA Kit, zgc:92555 ELISA Kit, adenylate cyclase activating polypeptide 1 ELISA Kit, adenylate cyclase activating polypeptide 1a ELISA Kit, adenylate cyclase activating polypeptide 1 (pituitary) ELISA Kit, adenylate cyclase activating polypeptide 1b ELISA Kit, ADCYAP1 ELISA Kit, Adcyap1 ELISA Kit, adcyap1a ELISA Kit, adcyap1 ELISA Kit, adcyap1b ELISA Kit
- 背景
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Gene Name: Adenylate Cyclase Activating Polypeptide 1, Pituitary
Gene Aliases: PACAP, PRP-48, Pituitary Adenylate Cyclase Activating Peptide
- 基因ID
- 116
- UniProt
- P18509
- 途径
- Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Activity, cAMP Metabolic Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Regulation of G-Protein Coupled Receptor Protein Signaling
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