Pituitary Adenylate Cyclase Activating Peptide ELISA 试剂盒
人 Pituitary Adenylate Cyclase Activating Peptide ELISA Kit, Colorimetric is an assay for quantification of 人 Pituitary Adenylate Cyclase Activating Peptide.
产品编号 ABIN5651435
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for Pituitary Adenylate Cyclase Activating Peptide ELISA 试剂盒 (ABIN5651435)
抗原
Pituitary Adenylate Cyclase Activating Peptide
适用
人
检测方法
Colorimetric
实验类型
Competition ELISA
检测范围
6.17 pg/mL - 500 pg/mL
应用范围
ELISA
样品类型
Plasma, Serum, Tissue Homogenate
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最低检测浓度
- 6.17 pg/mL
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原理
- Human Pituitary Adenylate Cyclase Activating Peptide (PACAP) ELISA Kit for detecting PACAP in serum, plasma, tissue homogenates
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). No significant cross-reactivity or interference between Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) and analogues was observed.
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灵敏度
- 2.92 pg/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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试剂未包括
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Pituitary Adenylate Cyclase Activating Peptide ELISA Kit
适用: 小鼠 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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样本量
- 50 μL
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实验时间
- 2 h
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板类型
- Pre-coated
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实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) and unlabeled Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) (Standards or samples) with the pre-coated antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the sample.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
限制
- 仅限研究用
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注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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储存条件
- 4 °C,-20 °C
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储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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有效期
- 12 months
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- Pituitary Adenylate Cyclase Activating Peptide
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背景
- Gene Aliases: ADCYAP1, PRP-48, Pituitary Adenylate Cyclase Activating Peptide
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基因ID
- 116
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UniProt
- P18509
抗原
-
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