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beta Actin ELISA 试剂盒

ACTB 适用: 人, 大鼠, 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
产品编号 ABIN5651365
发货至: 中国
  • 抗原 See all beta Actin (ACTB) ELISA试剂盒
    beta Actin (ACTB) (Actin, beta (ACTB))
    适用
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    人, 大鼠, 小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    1.56 ng/mL - 100 ng/mL
    最低检测浓度
    1.56 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Actin Beta (ACTb). No significant cross-reactivity or interference between Actin Beta (ACTb) and analogues was observed.
    灵敏度
    0.57 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Beta (ACTb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Beta (ACTb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Beta (ACTb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actin Beta (ACTb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Beta (ACTb) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Beta (ACTb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all beta Actin (ACTB) ELISA试剂盒
    beta Actin (ACTB) (Actin, beta (ACTB))
    别名
    Actin Beta (ACTB 产品)
    别名
    BRWS1 ELISA Kit, PS1TP5BP1 ELISA Kit, ACTA1 ELISA Kit, Actx ELISA Kit, E430023M04Rik ELISA Kit, beta-actin ELISA Kit, ps1tp5bp1 ELISA Kit, act-2 ELISA Kit, actb ELISA Kit, B-actin ELISA Kit, LOC100174883 ELISA Kit, LOC100304816 ELISA Kit, A ELISA Kit, ACT5C ELISA Kit, Ac5C ELISA Kit, Act ELISA Kit, Act-5C ELISA Kit, Act5 ELISA Kit, Act5c ELISA Kit, Actin ELISA Kit, Actin/BAP47 ELISA Kit, Actin5C ELISA Kit, BAP47 ELISA Kit, Bap47 ELISA Kit, CG4027 ELISA Kit, Dmel\\CG4027 ELISA Kit, M32055 ELISA Kit, T11 ELISA Kit, act ELISA Kit, act 5C ELISA Kit, act5C ELISA Kit, actin ELISA Kit, actin5C ELISA Kit, anon-EST:fe2D2 ELISA Kit, beta-actin/Bap47 ELISA Kit, cyt5C ELISA Kit, l(1)G0009 ELISA Kit, l(1)G0010 ELISA Kit, l(1)G0025 ELISA Kit, l(1)G0079 ELISA Kit, l(1)G0117 ELISA Kit, l(1)G0177 ELISA Kit, l(1)G0245 ELISA Kit, l(1)G0330 ELISA Kit, l(1)G0420 ELISA Kit, l(1)G0486 ELISA Kit, Bact ELISA Kit, ACT-5 ELISA Kit, ACTB ELISA Kit, B-ACTZF ELISA Kit, actba ELISA Kit, bact ELISA Kit, bactin1 ELISA Kit, bactzf ELISA Kit, wu:fd18f05 ELISA Kit, A26C1B ELISA Kit, POTE2alpha ELISA Kit, POTEACTIN ELISA Kit, Beta-actin ELISA Kit, actin beta ELISA Kit, actin, beta ELISA Kit, cytoplasmic actin OlCA1 ELISA Kit, beta-actin ELISA Kit, Actin 5C ELISA Kit, actin gamma 1 ELISA Kit, actin, beta 1 ELISA Kit, actin, beta L homeolog ELISA Kit, beta actin ELISA Kit, POTE ankyrin domain family member F ELISA Kit, actin, beta like 2 ELISA Kit, actin, cytoplasmic 1 ELISA Kit, ACTB ELISA Kit, Actb ELISA Kit, actb ELISA Kit, LOC100174883 ELISA Kit, LOC100304816 ELISA Kit, Act5C ELISA Kit, ACTG1 ELISA Kit, actb1 ELISA Kit, actb.L ELISA Kit, LOC100136352 ELISA Kit, POTEF ELISA Kit, ACTBL2 ELISA Kit, LOC109073280 ELISA Kit
    背景

    Gene Name: Actin Beta

    Gene Aliases: ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed

    途径
    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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