ACP5 ELISA 试剂盒
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- 抗原 See all ACP5 ELISA试剂盒
- ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Acid Phosphatase 5, Tartrate Resistant (ACP5). No significant cross-reactivity or interference between Acid Phosphatase 5, Tartrate Resistant (ACP5) and analogues was observed.
- 灵敏度
- 0.32 ng/mL
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Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA Kit
ACP5 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA KitACP5 适用: 大鼠 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA KitACP5 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA KitACP5 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA KitACP5 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA KitACP5 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Acid Phosphatase 5, Tartrate Resistant (ACP5) ELISA KitSmall Sample ACP5 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acid Phosphatase 5, Tartrate Resistant (ACP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acid Phosphatase 5, Tartrate Resistant (ACP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acid Phosphatase 5, Tartrate Resistant (ACP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acid Phosphatase 5, Tartrate Resistant (ACP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acid Phosphatase 5, Tartrate Resistant (ACP5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acid Phosphatase 5, Tartrate Resistant (ACP5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all ACP5 ELISA试剂盒
- ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))
- 别名
- Acid Phosphatase 5, Tartrate Resistant (ACP5 产品)
- 别名
- SPENCDI ELISA Kit, TRAP ELISA Kit, acp5 ELISA Kit, sb:cb576 ELISA Kit, zgc:63825 ELISA Kit, wu:fb19f01 ELISA Kit, wu:fb30b03 ELISA Kit, wu:fi14e01 ELISA Kit, wu:fj66f03 ELISA Kit, MGC78938 ELISA Kit, MGC89674 ELISA Kit, TR-AP ELISA Kit, zgc:92339 ELISA Kit, TRACP ELISA Kit, TTRRAP ELISA Kit, Trap ELISA Kit, UF ELISA Kit, acid phosphatase 5, tartrate resistant ELISA Kit, acid phosphatase 5a, tartrate resistant ELISA Kit, acid phosphatase 5, tartrate resistant S homeolog ELISA Kit, tartrate resistant acid phosphatase ELISA Kit, Tartrate-resistant acid phosphatase type 5 ELISA Kit, tartrate-resistant acid phosphatase type 5 ELISA Kit, acid phosphatase 5b, tartrate resistant ELISA Kit, ACP5 ELISA Kit, acp5a ELISA Kit, acp5.S ELISA Kit, acp5 ELISA Kit, NAEGRDRAFT_81291 ELISA Kit, ppa5 ELISA Kit, LOC100282899 ELISA Kit, acp5b ELISA Kit, Acp5 ELISA Kit
- 背景
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Gene Name: Acid Phosphatase 5, Tartrate Resistant
Gene Aliases: TRAP, TrATPase, Tartrate Resistant Acid Phosphatase, Type 5 acid phosphatase
- 基因ID
- 25732
- UniProt
- P29288
- 途径
- Transition Metal Ion Homeostasis
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