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Acetylcholinesterase ELISA 试剂盒

AChE 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
产品编号 ABIN5651345
发货至: 中国
  • 抗原 See all Acetylcholinesterase (AChE) ELISA试剂盒
    Acetylcholinesterase (AChE)
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    3.12 ng/mL - 200 ng/mL
    最低检测浓度
    3.12 ng/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Acetylcholinesterase (AchE). No significant cross-reactivity or interference between Acetylcholinesterase (AchE) and analogues was observed.
    灵敏度
    1.21 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetylcholinesterase (AchE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetylcholinesterase (AchE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetylcholinesterase (AchE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acetylcholinesterase (AchE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acetylcholinesterase (AchE) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acetylcholinesterase (AchE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all Acetylcholinesterase (AChE) ELISA试剂盒
    Acetylcholinesterase (AChE)
    别名
    Acetylcholinesterase (AChE 产品)
    别名
    ACEE ELISA Kit, ARACHE ELISA Kit, N-ACHE ELISA Kit, YT ELISA Kit, Ache ELISA Kit, GB14873 ELISA Kit, zgc:92550 ELISA Kit, ACE ELISA Kit, Dsim\\GD20515 ELISA Kit, GD20515 ELISA Kit, dsim_GLEANR_4292 ELISA Kit, ache ELISA Kit, mE1a ELISA Kit, mE1b ELISA Kit, mE1c ELISA Kit, mE1c-long ELISA Kit, mE1d ELISA Kit, mE1d' ELISA Kit, mE1e ELISA Kit, arache ELISA Kit, n-ache ELISA Kit, acetylcholinesterase (Cartwright blood group) ELISA Kit, acetylcholinesterase 2 ELISA Kit, acetylcholinesterase ELISA Kit, Acetylcholine esterase ELISA Kit, acetylcholinesterase (Cartwright blood group) L homeolog ELISA Kit, collagen type I alpha 2 chain ELISA Kit, ACHE ELISA Kit, AChE-2 ELISA Kit, Ache ELISA Kit, ache ELISA Kit, Dsim\Ace ELISA Kit, ache.L ELISA Kit, COL1A2 ELISA Kit, ACE-1 ELISA Kit
    背景

    Gene Name: Acetylcholinesterase

    Gene Aliases: ARAChE, N-AChE, YT

    基因ID
    43
    UniProt
    P22303
    途径
    Skeletal Muscle Fiber Development
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