Vasopressin ELISA 试剂盒
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- 抗原 See all Vasopressin (AVP) ELISA试剂盒
- Vasopressin (AVP) (Arginine Vasopressin (AVP))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 6.17 pg/mL - 500 pg/mL
- 最低检测浓度
- 6.17 pg/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
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This assay has high sensitivity and excellent specificity for detection of High Sensitive Antidiuretic Hormone (ADH).
No significant cross-reactivity or interference between High Sensitive Antidiuretic Hormone (ADH) and analogues was observed. - 灵敏度
- 2.65 pg/mL
- 质量等级
- High Sensitivity
- 组件
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- Pre-coated, ready to use 96-well strip plate
- Plate sealer for 96 wells
- Standard Diluent
- Assay Diluent A
- Assay Diluent B
- Stop Solution
- Standard
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Wash Buffer (30 x concentrate)
- Instruction manual
- 试剂未包括
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- Microplate reader with 450 nm filter.
- Precision single or multi-channel pipettes and disposable tips.
- Eppendorf Tubes for diluting samples.
- Deionized or distilled water.
- Absorbent paper for blotting the microtiter plate.
- Container for Wash Solution
- Featured
- Discover our best selling AVP ELISA Kit
- Top Product
- Discover our top product AVP ELISA Kit
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Arginine Vasopressin (AVP) ELISA Kit
AVP 适用: 小鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Arginine Vasopressin (AVP) ELISA KitAVP 适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Arginine Vasopressin (AVP) ELISA KitAVP 适用: 小鼠 Colorimetric Sandwich ELISA 1.25 pg/mL - 80 pg/mL Cell Culture Supernatant, Plasma, Serum
Arginine Vasopressin (AVP) ELISA KitAVP 适用: Various Species Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Arginine Vasopressin (AVP) ELISA KitAVP 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Arginine Vasopressin (AVP) ELISA KitAVP 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Arginine Vasopressin (AVP) ELISA KitAVP 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Arginine Vasopressin (AVP) ELISA KitAVP 适用: 绵羊 Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Arginine Vasopressin (AVP) ELISA KitAVP 适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Arginine Vasopressin (AVP) ELISA KitAVP 适用: 绵羊 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
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- 样本量
- 50 μL
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Antidiuretic Hormone (ADH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Antidiuretic Hormone (ADH) and unlabeled High Sensitive Antidiuretic Hormone (ADH) (Standards or samples) with the pre-coated antibody specific to High Sensitive Antidiuretic Hormone (ADH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Antidiuretic Hormone (ADH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Antidiuretic Hormone (ADH) in the sample.
- 实验流程
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- Prepare all reagents, samples and standards,
- Add 50μL standard or sample to each well.
And then add 50μL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37 °C, - Aspirate and wash 3 times,
- Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
- Aspirate and wash 5 times,
- Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
- Add 50μL Stop Solution. Read at 450 nm immediately.
- Prepare all reagents, samples and standards,
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Antidiuretic Hormone (ADH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Antidiuretic Hormone (ADH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
- 限制
- 仅限研究用
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- 注意事项
- The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
- 注意事项
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. - 储存条件
- 4 °C/-20 °C
- 储存方法
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- For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
- For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit. - For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
- 有效期
- 6 months
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- 抗原 See all Vasopressin (AVP) ELISA试剂盒
- Vasopressin (AVP) (Arginine Vasopressin (AVP))
- 别名
- Antidiuretic Hormone (ADH) (AVP 产品)
- 别名
- ADH ELISA Kit, ARVP ELISA Kit, AVP-NPII ELISA Kit, AVRP ELISA Kit, VP ELISA Kit, 2610201A18Rik ELISA Kit, Akr1a4 ELISA Kit, Vp ELISA Kit, Vsp ELISA Kit, DI ELISA Kit, Vas ELISA Kit, Adh ELISA Kit, Adh1a ELISA Kit, Adh1c ELISA Kit, adh ELISA Kit, arvp ELISA Kit, avrp ELISA Kit, avp-npii ELISA Kit, copeptin ELISA Kit, vasotocin ELISA Kit, arginine vasopressin ELISA Kit, aldo-keto reductase family 1, member A1 (aldehyde reductase) ELISA Kit, alcohol dehydrogenase 1 (class I) ELISA Kit, AVP ELISA Kit, Akr1a1 ELISA Kit, Avp ELISA Kit, Adh1 ELISA Kit, avp ELISA Kit
- 途径
- cAMP Metabolic Process
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