LDHB ELISA 试剂盒
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北京 101111
Quick Overview for LDHB ELISA 试剂盒 (ABIN5564579)
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See all LDHB ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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Analytical Method
- Quantitative
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试剂未包括
- Microplate reader capable of measuring absorbance at 405 nm. Pipettes (1-20 µL, 20-200 µL, and multiple channel). Deionized or distilled reagent grade water Incubator (37 °C)
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Lactate Dehydrogenase B (LDHB) ELISA Kit
LDHB 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Lactate Dehydrogenase B (LDHB) ELISA KitLDHB 适用: 人 Colorimetric Sandwich ELISA 23.5 mU/mL - 1500 mU/mL Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate, Urine
Lactate Dehydrogenase B (LDHB) ELISA KitLDHB 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lactate Dehydrogenase B (LDHB) ELISA KitLDHB 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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实验时间
- 4 h
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板类型
- Pre-coated
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结果分析
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- Calculate the mean value of the duplicate or triplicate readings for each standard and sample.
- To generate a standard curve, plot the graph using the standard concentrations on the x-axis and the corresponding mean 450 nm absorbance (OD) on the y-axis. The best-fit line can be determined by regression analysis using log-log or four-parameter logistic curve-fit.
- Determine the unknown sample concentration from the standard curve and multiply the value by the dilution factor.
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限制
- 仅限研究用
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注意事项
- This product is for Research Use Only and is Not For Use In Diagnostic Procedures. Prepare all reagents (working diluent buffer, wash buffer, standard, biotinylated antibody, and SP Conjugate) as instructed, prior to running the assay. Prepare all samples prior to running the assay. The dilution factors for the samples are suggested in this insert. However, the user should determine the optimal dilution factor. Spin down the SP Conjugate vial and the biotinylated antibody vial before opening and using contents. The Stop Solution is an acidic solution. The kit should not be used beyond the expiration date. 2
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储存条件
- 4 °C,-20 °C
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- LDHB (Lactate Dehydrogenase B (LDHB))
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别名
- L-Lactate Dehydrogenase B Chain (LDH-B)
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基因ID
- 3945
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UniProt
- P07195
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途径
- Warburg Effect
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