LIF ELISA 试剂盒
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Quick Overview for LIF ELISA 试剂盒 (ABIN5526766)
抗原
See all LIF ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.64 pg/mL
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原理
- This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human LIF.
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Analytical Method
- Quantitative
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特异性
- LIF
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组件
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- Plate
- Standard
- Diluent
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试剂未包括
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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Leukemia Inhibitory Factor (LIF) ELISA Kit
LIF 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Bronchoalveolar Lavage, Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: 小鸡 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor (LIF) ELISA KitLIF 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- LIF (Leukemia Inhibitory Factor (LIF))
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别名
- LIF
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背景
- Leukemia inhibitory factor (LIF) is an interleukin 6 family cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. Cells known to express LIF include activated T-cells, monocytes, and astrocytes, osteoblasts, keratinocytes, mast cells, and fibroblasts. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. During pregnancy LIF is involved in decidualization of the maternal endometrium and implantation of the blastocyst to the endometrium. Women with decreased production of LIF and other cytokines are fertile and able to become pregnant, but there is an increased risk for unexplained, recurrent miscarriages. It has been suggested that recombinant human LIF might help to improve the implantation rate in women with unexplained infertility. In addition, LIF is typically added to stem cell culture medium to reduce spontaneous differentiation.
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基因ID
- 3976
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NCBI登录号
- NM_001257135
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UniProt
- P15018
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途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
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