Glucagon ELISA 试剂盒
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Quick Overview for Glucagon ELISA 试剂盒 (ABIN5526763)
抗原
See all Glucagon (GCG) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 8.42 pg/mL
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原理
- This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of Glucagon.
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Analytical Method
- Quantitative
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特异性
- Glucagon
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组件
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- Plate
- Standard
- Diluent
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试剂未包括
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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Glucagon (GCG) ELISA Kit
GCG 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Glucagon (GCG) ELISA KitGCG 适用: 小鼠 Colorimetric Competition ELISA 18.52 pg/mL - 1500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glucagon (GCG) ELISA KitGCG 适用: 人 Colorimetric Competition ELISA 18.52 pg/mL - 1500 pg/mL Plasma, Serum
Glucagon (GCG) ELISA KitGCG 适用: 大鼠 Colorimetric Competition ELISA 18.52 pg/mL - 1500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glucagon (GCG) ELISA KitGCG 适用: 人 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Glucagon (GCG) ELISA KitGCG 适用: Pig Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Glucagon (GCG) ELISA KitGCG 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Glucagon (GCG) ELISA KitGCG 适用: 小鸡 Colorimetric Competition ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Glucagon (GCG) ELISA KitGCG 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Glucagon (GCG) ELISA KitGCG 适用: 小鼠 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- Glucagon (GCG)
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别名
- Glucagon
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背景
- Glucagon is a 29-amino acid polypeptide, produced by alpha cells of the pancreas. The polypeptide has a molecular weight of 3485 Da that plays a critical role in glucose metabolism and homeostasis. It works to raise the concentration of glucose in the bloodstream. It is also used as a medication to treat a number of health conditions. Its effect is opposite to that of insulin, which lowers the glucose. The pancreas releases glucagon when the concentration of glucose in the bloodstream falls too low. Glucagon causes the liver to convert stored glycogen into glucose, which is released into the bloodstream. High blood-glucose levels, on the other hand, stimulate the release of insulin. Glucagon allows glucose to be taken up and used by insulin-dependent tissues. Thus, glucagon and insulin are part of a feedback system that keeps blood glucose levels stable. Abnormally elevated levels of glucagon may be caused by pancreatic tumors, such as glucagonoma, symptoms of which include necrolytic migratory erythema, reduced amino acids, and hyperglycemia. It may occur alone or in the context of multiple endocrine neoplasia type 1.
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基因ID
- 2641
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NCBI登录号
- NM_002054
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UniProt
- P01275
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途径
- Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Negative Regulation of intrinsic apoptotic Signaling
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