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NFYA ELISA 试剂盒

NFYA 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
产品编号 ABIN5526700
发货至: 中国
  • 抗原 See all NFYA ELISA试剂盒
    NFYA (Nuclear Transcription Factor Y, alpha (NFYA))
    适用
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    DNA-Binding ELISA
    应用范围
    ELISA
    原理
    Human NF-YA Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical NF-YA binding sequences to semi-quantitatively detect active NF-YA in lysates or nuclear extracts. Only available in North America.
    样品类型
    Cell Lysate, Nuclear Extract
    Analytical Method
    Semi-Quantitative
    特异性
    The olionucleotide/antibody pair provided in this kit recognizes mouse NFYA in whole lysates and nuclear extracts.
    产品特性
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    组件
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    有效期
    6 months
  • 抗原 See all NFYA ELISA试剂盒
    NFYA (Nuclear Transcription Factor Y, alpha (NFYA))
    别名
    NF-YA (NFYA 产品)
    别名
    wu:fb16e03 ELISA Kit, ik:tdsubc_2b6 ELISA Kit, xx:tdsubc_2b6 ELISA Kit, NFYA ELISA Kit, CBF-A ELISA Kit, CBF-B ELISA Kit, HAP2 ELISA Kit, NF-YA ELISA Kit, AA407810 ELISA Kit, Cbf-b ELISA Kit, SEZ-10 ELISA Kit, SEZ10 ELISA Kit, nuclear transcription factor Y, alpha ELISA Kit, nuclear transcription factor Y subunit alpha ELISA Kit, nuclear transcription factor-Y alpha ELISA Kit, nfya ELISA Kit, NFYA ELISA Kit, Nfya ELISA Kit
    基因ID
    4800
    UniProt
    P23511
    途径
    Regulation of Lipid Metabolism by PPARalpha
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