Estradiol ELISA 试剂盒
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北京 101111
Quick Overview for Estradiol ELISA 试剂盒 (ABIN5524164)
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See all Estradiol ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 12.5 pg/mL
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原理
- Bovine E2 (Estradiol) ELISA Kit
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Analytical Method
- Quantitative
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特异性
- Specifically binds with E2 , no obvious cross reaction with other analogues.
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灵敏度
- 7.5 pg/mL
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组件
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- ELISA Microplate(Dismountable)
- Lyophilized Standard
- Lyophilized Biotin-labeled Antibody(Concentrated)
- Biotin-labeled Antibody
- HRP-Streptavidin Conjugate(SABC
- 100X)
- TMB Substrate
- Sample Dilution Buffer
- Stop Solution
- Plate Sealer
- Product Description
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试剂未包括
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- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Precision single (0.5-10μL
- 5-50μL
- 20-200μL
- 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
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Estradiol ELISA Kit
适用: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Estradiol ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Estradiol ELISA Kit适用: Cow Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Estradiol ELISA Kit适用: Various Species Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Estradiol ELISA KitSmall Sample 适用: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Estradiol ELISA KitQuick 适用: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Estradiol ELISA Kit适用: Various Species Colorimetric Sandwich ELISA Fecal, Tissue Culture Medium, Urine
Estradiol ELISA Kit适用: 大鼠 Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Estradiol ELISA Kit适用: 绵羊 Colorimetric Competition ELISA 40-1400 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Competitive ELISA, Coated with Antigen
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样本量
- 50 μL
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实验时间
- 2 h
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板类型
- Pre-coated
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实验流程
- Step 1: Wash the plate twice before adding the standard and sample. Step 2: Add 50 µL standard or sample into each well. Immediately add 50 µL biotin-labeled antibody working solution into each well(The tip can't be reused after touching the liquid.), gently shake the plate for 1 min, seal closely and statically incubate for 45 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution into each well, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.
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限制
- 仅限研究用
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注意事项
- Avoid direct light exposure to antibody solutions
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储存条件
- 4 °C
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储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
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有效期
- 12 months
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: "Reproductive fecundity of Iraqi Awassi ewes immunized against synthetic inhibin-α subunit or steroid-free bovine follicular fluid." in: Asian-Australasian journal of animal sciences, Vol. 31, Issue 8, pp. 1169-1175, (2018) (PubMed).
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- Estradiol
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背景
- Estradiol, E2
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