NLRP1 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for NLRP1 ELISA 试剂盒 (ABIN5520075)
抗原
See all NLRP1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 7.813 pg/mL
-
原理
- Mouse NLRP1 (NLR Family, Pyrin Domain Containing 1) ELISA Kit
-
Analytical Method
- Quantitative
-
特异性
- Specifically binds with NLRP1 , no obvious cross reaction with other analogues.
-
灵敏度
- 4.688 pg/mL
-
组件
-
- ELISA Microplate(Dismountable)
- Lyophilized Standard
- Biotin-labeled Antibody
- HRP-Streptavidin Conjugate(SABC
- 100X)
- TMB Substrate
- Sample Dilution Buffer
- Stop Solution
- Plate Sealer
- Product Description
-
试剂未包括
-
- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Precision single (0.5-10μL
- 5-50μL
- 20-200μL
- 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
-
-
NLR Family, Pyrin Domain Containing 1 (NLRP1) ELISA Kit
NLRP1 适用: 人 Colorimetric Sandwich ELISA 18.75 pg/mL - 1200 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
NLR Family, Pyrin Domain Containing 1 (NLRP1) ELISA KitNLRP1 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
Sandwich ELISA, Double Antibody
-
样本量
- 100 μL
-
实验时间
- 4 h
-
板类型
- Pre-coated
-
实验流程
- Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.
-
限制
- 仅限研究用
-
-
-
注意事项
- Avoid direct light exposure to antibody solutions
-
储存条件
- 4 °C
-
储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
-
有效期
- 12 months
-
-
- NLRP1 (NLR Family, Pyrin Domain Containing 1 (NLRP1))
-
别名
- NLRP1
-
背景
- NACHT, LRR and PYD domains-containing protein 1a, Caspase recruitment domain-containing protein 7, Death effector filament-forming ced-4-like apoptosis protein, Nucleotide-binding domain and caspase recruitment domain, Nlrp1a, Card7, Nalp1, Nalp1a, Nlrp1
-
UniProt
- Q2LKU9
-
途径
- Positive Regulation of Endopeptidase Activity, Inflammasome
抗原 See all NLRP1 ELISA试剂盒
-
