SAA ELISA 试剂盒
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Quick Overview for SAA ELISA 试剂盒 (ABIN5519845)
抗原
See all SAA ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 9.375 ng/mL
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原理
- For quantitative detection of SAA in serum, plasma, tissue homogenates.
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Analytical Method
- Quantitative
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特异性
- This assay has high sensitivity and excellent specificity for detection of SAA. No significant cross-reactivity or interference between SAA and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between SAA and all the analogues, therefore, cross reaction may still exist.
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灵敏度
- 5.625 ng/mL
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组件
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- Pre-coated, ready to use 96-well strip plate
- Plate sealer for 96 wells
- Standard
- Sample/Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate (SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25 x concentrate)
- Preprocess Agent A
- Preprocess Agent B
- Instruction manual
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Serum Amyloid A (SAA) ELISA Kit
SAA 适用: 人 Colorimetric Competition ELISA 39.06 ng/mL - 10000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Serum Amyloid A (SAA) ELISA KitSAA 适用: 山羊 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Serum Amyloid A (SAA) ELISA KitSAA 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Serum Amyloid A (SAA) ELISA KitSAA 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Serum Amyloid A (SAA) ELISA KitSAA 适用: Cow Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Serum Amyloid A (SAA) ELISA KitSAA 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum
Serum Amyloid A (SAA) ELISA KitSAA 适用: 犬 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Serum Amyloid A (SAA) ELISA KitSAA 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Serum Amyloid A (SAA) ELISA KitSAA 适用: 小鸡 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Serum Amyloid A (SAA) ELISA KitSAA 适用: 绵羊 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Sandwich ELISA, Double Antibody
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样本量
- 100 μL
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板类型
- Pre-coated
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样品制备
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The user should estimate the concentration of target protein in the test sample, and select a proper dilution factor to make the diluted target protein concentration fall in the optimal detection range of the kit. Dilute the sample with the provided dilution buffer, and several trials may be necessary. The test sample must be well mixed with the dilution buffer. And also standard curves and sample should be making in pre-experiment. If samples with very high concentrations, dilute samples with PBS first and then dilute the samples with Sample Dilution. The matrix components in the sample will affect the test results, which it need to be diluted at least 1/2 with Sample Dilution Buffer before testing!
In order to reduce the influence of the substrate on the experiment, we suggest preprocess the sample.- Serum/Plasma: Add 30ul of Preprocess Agent A to 60ul Serum/Plasma samples, mix well, incubate 10 minutes at room temperature. Then add 30ul of Preprocess Agent B, mix well. Note: The matrix components in the sample will affect the test results. After sample pretreatment, it is still necessary to dilute at least 1/2 with Sample Dilution Buffer before testing! The sample dilution caused by pretreatment should be considered in the calculation of the result.
- Cell Culture Supernatant: Add 20ul of Preprocess Agent A to 100ul samples, mix well, incubate 10 minutes at room temperature. Then add 20ul of Preprocess Agent B, mix well. At last, add 60ul Sample dilution buffer, mix well and test. Note: Sample is diluted for 2 fold dilution (1/2).The sample dilution caused by pretreatment should be considered in the calculation of the result.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
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- For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
- For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
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有效期
- 6 months
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- SAA (Serum Amyloid A (SAA))
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别名
- SAA
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背景
- SAA(Serum Amyloid A)
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UniProt
- P35541
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