SAA1 ELISA 试剂盒
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Quick Overview for SAA1 ELISA 试剂盒 (ABIN5510706)
抗原
See all SAA1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.56 ng/mL
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原理
- Sandwich Human SAA/SAA1 ELISA Kit to quantitate Human SAA1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: S20-Y122Capture antibody and Detection antibody: monoclonal antibody from mouse, monoclonal antibody from mouse
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交叉反应 （详细）
- No significant cross-reactivity or interference with mouse SAA1 was observed. This claim is limited by existing techniques, therefore, cross-reactivity may exist between SAA1 and its analogs.
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灵敏度
- < 150 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Serum Amyloid A1 (SAA1) ELISA Kit
SAA1 适用: 人 Colorimetric Sandwich ELISA 156 ng/mL - 10000 ng/mL Serum, Urine
Serum Amyloid A1 (SAA1) ELISA KitSAA1 适用: 猴 Colorimetric Competition ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Serum Amyloid A1 (SAA1) ELISA KitSAA1 适用: 绵羊 Colorimetric Competition ELISA 1.875 ng/mL - 120 ng/mL Plasma, Serum, Tissue Homogenate
Serum Amyloid A1 (SAA1) ELISA KitSAA1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312-20 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Serum Amyloid A1 (SAA1) ELISA KitSAA1 适用: 小鼠 AA 20-122 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Serum Amyloid A1 (SAA1) ELISA KitSAA1 适用: 猴 Colorimetric Sandwich ELISA 0.625-40 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.6%, 4.4%, 5%
Inter-Assay Precision (CV%): 9.3%, 6.3%, 5.9% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- SAA1 (Serum Amyloid A1 (SAA1))
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别名
- SAA1
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背景
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Background: Serum amyloid A protein (SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.
Gene Full Name: serum amyloid A1
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基因ID
- 6288
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UniProt
- P0DJI8
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途径
- Toll-Like Receptors Cascades
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