SERPINA7 ELISA 试剂盒
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Quick Overview for SERPINA7 ELISA 试剂盒 (ABIN5510681)
抗原
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human Serpin A7 ELISA Kit to quantitate Human SERPINA7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: A21-A415Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from sheep
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Serpin Family A Member 7 (SERPINA7) ELISA Kit
SERPINA7 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Serpin Family A Member 7 (SERPINA7) ELISA KitSERPINA7 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Serpin Family A Member 7 (SERPINA7) ELISA KitSERPINA7 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Serpin Family A Member 7 (SERPINA7) ELISA KitSERPINA7 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Serpin Family A Member 7 (SERPINA7) ELISA KitSERPINA7 适用: 人 Colorimetric Sandwich ELISA 781 pg/mL - 50000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Serpin Family A Member 7 (SERPINA7) ELISA KitSERPINA7 适用: 人 Colorimetric Competition ELISA
Serpin Family A Member 7 (SERPINA7) ELISA KitSERPINA7 适用: 人 Colorimetric Competition ELISA 12.5-200 ng/mL Plasma, Serum, Tissue Homogenate
Serpin Family A Member 7 (SERPINA7) CLIA KitSERPINA7 适用: 人 Chemiluminescent Sandwich ELISA 0.14 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Serpin Family A Member 7 (SERPINA7) ELISA KitSERPINA7 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 6.1%, 5.3%, 5.2%
Inter-Assay Precision (CV%): 7.3%, 5.4%, 5.8% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- SERPINA7 (Serpin Family A Member 7 (SERPINA7))
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别名
- SERPINA7
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背景
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Background: Thyroxine-binding globulin (TBG), also known as SERPINA7, is the major thyroid hormone transport protein in serum. There are three proteins including thyroxine-binding globulin (TBG), transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3'-triiodothyronine (T3) in the bloodstream. This gene belongs to the serpin family in genomics, but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined. Using DNA blot hybridization, it is detected that there is a single-copy TBG gene located on the long arm of the X chromosome.
Gene Full Name: serpin family A member 7
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基因ID
- 6906
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UniProt
- P05543
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途径
- Hormone Transport
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