ORM1 ELISA 试剂盒
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Quick Overview for ORM1 ELISA 试剂盒 (ABIN5510656)
抗原
See all ORM1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.56 ng/mL
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原理
- Sandwich Human AGP1/alpha 1 acid glycoprotein ELISA Kit to quantitate Human ORM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: Q19-S201Capture antibody and Detection antibody: a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 150 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Orosomucoid 1 (ORM1) ELISA Kit
ORM1 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Orosomucoid 1 (ORM1) ELISA KitORM1 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Orosomucoid 1 (ORM1) ELISA KitORM1 适用: Cow Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Plasma, Serum, Urine
Orosomucoid 1 (ORM1) ELISA KitORM1 适用: Cow Colorimetric Competition ELISA 62.5 ng/mL - 4000 ng/mL Plasma, Serum, Tissue Homogenate
Orosomucoid 1 (ORM1) ELISA KitORM1 适用: 人 Colorimetric Competition ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
Orosomucoid 1 (ORM1) ELISA KitORM1 适用: 小鸡 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Orosomucoid 1 (ORM1) ELISA KitORM1 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Orosomucoid 1 (ORM1) ELISA KitORM1 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Orosomucoid 1 (ORM1) ELISA KitORM1 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Orosomucoid 1 (ORM1) ELISA KitORM1 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 6.1%, 7.7%, 4.6%
Inter-Assay Precision (CV%): 7%, 9.3%, 5.8% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- ORM1 (Orosomucoid 1 (ORM1))
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别名
- ORM1
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背景
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Background: Alpha-1-acid glycoprotein, or orosomucoid, is a relatively abundant plasma protein synthesized in the liver. It has a molecular weith of 40,000 Da and is a single polypeptide chain of about 180 amino acids. AGP is considered an acute phase reactant, its plasma levels increase several fold during acute infections. It belongs to the lipocalin family and binds numerous basic and neutral lipophilic drugs and steroid hormones. And this gene is located in 9q34.3-qter.
Gene Full Name: orosomucoid 1
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基因ID
- 5004
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UniProt
- P02763
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途径
- Response to Growth Hormone Stimulus
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