MUC16 ELISA 试剂盒
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Quick Overview for MUC16 ELISA 试剂盒 (ABIN5510649)
抗原
See all MUC16 (CA125) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 3.12 U/mL
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原理
- Sandwich Human CA125/MUC16 ELISA Kit to quantitate Human MUC16 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.
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Analytical Method
- Quantitative
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特异性
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Natural protein
Capture antibody and Detection antibody: a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 1.5 U/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Mucin 16, Cell Surface Associated (CA125) ELISA Kit
CA125 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mucin 16, Cell Surface Associated (CA125) ELISA KitCA125 适用: 人 Colorimetric Sandwich ELISA 1.56 U/mL - 100 U/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Mucin 16, Cell Surface Associated (CA125) ELISA KitCA125 适用: 人 Colorimetric Sandwich ELISA 1.563 IU/mL - 100 IU/mL Plasma, Serum, Tissue Homogenate
Mucin 16, Cell Surface Associated (CA125) ELISA KitCA125 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 IU/mL - 200 IU/mL Plasma, Serum, Tissue Homogenate
Mucin 16, Cell Surface Associated (CA125) ELISA KitCA125 适用: 大鼠 Colorimetric Sandwich ELISA 7.813 IU/mL - 500 IU/mL Plasma, Serum, Tissue Homogenate
Mucin 16, Cell Surface Associated (CA125) ELISA KitCA125 适用: 人 Colorimetric Sandwich ELISA 1.56 U/mL - 100 U/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Mucin 16, Cell Surface Associated (CA125) ELISA KitCA125 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7%, 7.9%, 2.7%
Inter-Assay Precision (CV%): 7%, 7%, 7.8% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "TGFβ1 in fibroblasts-derived exosomes promotes epithelial-mesenchymal transition of ovarian cancer cells." in: Oncotarget, Vol. 8, Issue 56, pp. 96035-96047, (2017) (PubMed).
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- MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))
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别名
- MUC16
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背景
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Background: CA-125, also known as mucin 16 or MUC16, is a protein that in humans is encoded by the MUC16 gene. MUC16 is a member of the mucin family glycoproteins. It is a component of the ocular surface, the respiratory tract and the female reproductive tract epithelia. Since MUC16 is highly glycosylated it creates a hydrophilic environment that acts as a lubricating barrier against foreign particles and infectious agents on the apical membrane of epithelial cells. Also, the cytoplasmic tail of MUC16 has been shown to interact with cytoskeleton by binding members of the ERM protein family. The expression of mucin 16 has been shown to be altered in dry eye, cystic fibrosis, and several types of cancers.
Gene Full Name: mucin 16, cell surface associated
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基因ID
- 94025
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UniProt
- Q8WXI7
抗原 See all MUC16 (CA125) ELISA试剂盒
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