Interleukin 17a ELISA 试剂盒
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Quick Overview for Interleukin 17a ELISA 试剂盒 (ABIN5068352)
抗原
See all Interleukin 17a (IL17A) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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Analytical Method
- Quantitative
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灵敏度
- < 5 pg/mL
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产品特性
- Interleukin 17A (IL-17A) Human, ELISA Kit (Cytotoxic T-lymphocyte-associated Antigen 8, CTLA-8, CTLA8)
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组件
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- IL-17A microtiter plate: 1x96 wells
- Biotin conjugate (Anti-human IL-17A polyclonal antibody conjugated to Biotin): 1x7ml
- Avidin conjugate (Avidin conjugated to HRP): 1x12ml
- IL-17A standard (Recombinant human IL-17A) (2000pg/vial): 1x2 vials
- Calibrator diluent 1: 1x25ml
- Calibrator diluent 2: 1x25ml
- Washer buffer (20X): 1x60ml
- Substarte A (Buffered solution with 2): 1x10ml
- Substrate B (Buffered solution with TMB): 1x10ml
- Stop Solution (O4): 1x14ml
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Interleukin 17A (IL17A) ELISA Kit
IL17A 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 AA 22-158 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 人 AA 20-155 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 Colorimetric Sandwich ELISA 6-600 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 大鼠 Colorimetric Sandwich ELISA 3.13-200 pg/mL Cell Culture Supernatant, Plasma, Serum
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板类型
- Pre-coated
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实验流程
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Principle:
- This IL-17A enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-17A. Standards or samples are then added to the appropriate microtiter plate wells. A biotin-conjugated antibody preparation specific for IL-17A was added and incubated. IL-17A, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound IL-17A and other components of the sample. Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin, and a TMB (3,3&
- 39,5, 5&
- 39, tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-17A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- -20°C
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- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
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别名
- Interleukin 17A (IL-17A)
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背景
- Interleukin -17A (IL-17A) is a secreted, homodimeric glycoprotein linked by disulfide link with a molecular mass of 35kD. The cytokine is the proto-type of a newly discovered pro-inflammatory cytokine family which consists of IL-17A, IL-17B, IL-17C, IL- 17D, IL-17E (IL-25) and IL-17F. All the IL-17s have similar structure with four highly conserved cysteine residues and same 3 dimensional structure, which distinguish this family from other cytokines.
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