Mesothelin ELISA 试剂盒
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- 抗原 See all Mesothelin (MSLN) ELISA试剂盒
- Mesothelin (MSLN)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 样品类型
- Cell Culture Lysate, Plasma, Serum
- Analytical Method
- Quantitative
- 灵敏度
- 0.010 ng/mL
- 产品特性
- Mesothelin, ELISA Kit (Human) (CAK1 Antigen, Pre-pro-megakaryocyte-potentiating Factor, MSLN, MPF)
- 组件
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- Mesothelin Microplate: 12 strips x 8 wells
- Mesothelin monoclonal antibody, conjugated to HRP: 1 x 21ml
- Mesothelin Standard (lyophilized): 1 x 100ng
- Assay Diluent R 1 x 11ml
- Calibrator Diluent R Concentrate: 1 x 21ml
- Wash Buffer Concentrate: 1 x 21ml
- Color Reagent A (stabilized hydrogen peroxide): 1 x 12.5ml
- Color Reagent B (tetramethylbenzidine): 1 x 12.5ml
- Stop Solution (2N sulfuric acid): 1 x 6ml
- Plate Covers: 4 adhesive strips
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Mesothelin (MSLN) ELISA Kit
MSLN 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mesothelin (MSLN) ELISA KitMSLN 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mesothelin (MSLN) ELISA KitMSLN 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Mesothelin (MSLN) ELISA KitMSLN 适用: 人 AA 296-580 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
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- 板类型
- Pre-coated
- 实验流程
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Principle:
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mesothelin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Mesothelin present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for Mesothelin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Mesothelin bound in the initial step. The color development is stopped and the intensity of the color is measured.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- 4°C
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- 抗原 See all Mesothelin (MSLN) ELISA试剂盒
- Mesothelin (MSLN)
- 别名
- Mesothelin (MSLN 产品)
- 别名
- MPF ELISA Kit, SMRP ELISA Kit, MSLN ELISA Kit, mesothelin ELISA Kit, MSLN ELISA Kit, Msln ELISA Kit, LOC611363 ELISA Kit, LOC100524016 ELISA Kit
- 背景
- Mesothelin, also known as CAK1 and ERC, is a protein that is mainly expressed in the mesothelial cells lining the pleura, pericardium, and peritoneum. The Mesothelin gene encodes a precursor protein of 70kD that is cleaved into two products, including megakaryocyte potentiating factor (aa37-286) and Mesothelin (aa296-606). Megakaryocyte potentiation factor is a secreted protein of about 32kD, and it functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes.
- 途径
- EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
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