Serotonin ELISA 试剂盒
Quick Overview for Serotonin ELISA 试剂盒 (ABIN5068229)
抗原
See all Serotonin (5-HT) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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Analytical Method
- Quantitative
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灵敏度
- 0.08-19.8 ng/mL
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产品特性
- Serotonin, Human (5-Hydroxytryptamine, 5HT, 5-HT, 5-HT5A, 5-HT-5A) ELISA Kit
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组件
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- Microtiter Plate, 1x96 wells
- Serotonin Pab 1x8ml
- Serotonin (Biotin) Pab, 1x8ml
- Streptavidin (AP), 100x, 1x300ul
- Standard, 0ng/ml 1x1ml
- Standard, 0.08ng/ml 1x1ml
- Standard, 0.24ng/ml 1x1ml
- Standard, 0.73ng/ml 1x1ml
- Standard, 2.2ng/ml 1x1ml
- 0: Standard, 6.6ng/ml 1x1ml
- 1: Standard, 19.8ng/ml 1x1ml
- 2: Control 1, ng/ml, 1x500ul
- 3: Control 2, ng/ml, 1x500ul
- 4: Acylation Reagent, 1x3ml
- 5: Assay Buffer, 10x, 1x50ml
- 6: Wash Buffer, 20x, 2x50ml
- 7: Substrate (PNPP), 2x13ml
- 8: Stop Solution (1N NaOH), 1x15ml
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5-Hydroxytryptamine (5-HT) ELISA Kit
5-HT 适用: Various Species Colorimetric Competition ELISA 11.11 ng/mL - 900 ng/mL Plasma, Serum
5-Hydroxytryptamine (5-HT) ELISA Kit5-HT 适用: Various Species Colorimetric Competition ELISA 15.63 ng/mL - 1000 ng/mL Cell Culture Supernatant, Plasma, Serum
5-Hydroxytryptamine (5-HT) ELISA Kit5-HT 适用: Various Species Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
5-Hydroxytryptamine (5-HT) ELISA Kit5-HT 适用: 小鼠 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
5-Hydroxytryptamine (5-HT) ELISA Kit5-HT 适用: 人 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
5-Hydroxytryptamine (5-HT) ELISA Kit5-HT 适用: 大鼠 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
5-Hydroxytryptamine (5-HT) ELISA Kit5-HT 适用: 绵羊 Colorimetric Competition ELISA 0.625 ng/mL - 10 ng/mL Plasma, Serum
5-Hydroxytryptamine (5-HT) ELISA Kit5-HT 适用: 大鼠 Colorimetric Competition ELISA 0.8-160 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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实验流程
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Principle:
- The sample preparation (derivatization of serotonin to N-acylserotonin) is part of the sample dilution and is achieved by incubation of the respective sample with the Acylation Reagent.
- The assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and a non-biotinylated antigen for a fixed number of antibody binding sites. The amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of anti-biotin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is achieved by comparing the enzymatic activity of unknowns with a response curve prepared by using known standards.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C
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- Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))
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别名
- Serotonin
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物质类
- Chemical
抗原 See all Serotonin (5-HT) ELISA试剂盒
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