IgG3 ELISA 试剂盒
Quick Overview for IgG3 ELISA 试剂盒 (ABIN5068222)
抗原
See all IgG3 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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灵敏度
- 25 ng/mL
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产品特性
- IgG3, Mouse, ELISA Kit
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组件
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- Anti-Mouse IgG3 Microwell Strip Plate: 12X8-well strips
- Mouse IgG3 Control: 1X0.65ml
- Mouse IgG3 Standard (50ng/ml): 1X0.65ml
- Mouse IgG3 Standard (100ng/ml): 1X0.65ml
- Mouse IgG3 Standard (400ng/ml): 1X0.65ml
- Mouse IgG3 Standard (400ng/ml): 1X0.65ml
- Mouse IgG3 Standard (1000ng/ml): 1X0.65ml
- Anti-Mouse IgG3 HRP Conjugate (100X): 1X0.15ml
- Sample Diluent Concentrate (20X): 1X10ml
- Wash Solution Concentrate (100X): 1X10ml
- TMB Substrate: 1X12ml
- Stop Solution: 1X12ml
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IgG3 ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG3 ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgG3 ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
IgG3 ELISA Kit适用: 人 Colorimetric Competition ELISA 2.7-2000 ng/mL Plasma, Serum
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实验流程
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Principle:
- The Mouse IgG3 ELISA kit is based on the binding of mouse IgG3 in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgG3 present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgG3 in samples and control is calculated from a curve of standards containing known concentrations of IgG3.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C
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- IgG3
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物质类
- Antibody
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背景
- Immunoassays using heavy-chain specific antibodies provide for selective, sensitive quantification of mouse immunoglobulins IgG (and subclasses), IgA and IgM, as found circulating in blood or as present in other body fluids, including saliva, milk/colostrum, ascites, tears and mucosa of linings of the gut, respiratory or urigenital tracts.
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