Lactoferrin ELISA 试剂盒
Quick Overview for Lactoferrin ELISA 试剂盒 (ABIN5068137)
抗原
See all Lactoferrin (LTF) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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产品特性
- Lactoferrin, Human Plasma ELISA Kit,
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组件
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- Sample Dilution Buffer: PBS, BSA, Tween-20 and 0.01% thimerosal, 3 x 25ml
- LTF Standard: Approximately 300ng of purified LTF with BSA in lyophilized form, 1x1vial
- Wash Buffer (20x), Tris-HCl buffer containing NaCl, Tween-20, and 0.01% thimerosal. 1x100ml
- LTF Antibody, Concentrated solution of monoclonal antibody to LTF in phosphate buffer containing NaCl, BSA, glycerol, and 0.01% thimerosal. 1x75ul
- Streptavidin-HRP, Concentrated solution of streptavidin-coupled HRP in Tris-HCl buffer containing BSA and 0.01% thimerosal. 1x75ul
- Dilution Buffer, PBS, BSA, Tween-20, and 0.01% thimerosal, 3x8ml
- OPD Diluting Buffer, Citrate buffer containing 2 and 0.01% thimerosal. 1x20ml
- Stop Solution, 1M O4. 1x20ml
- OPD, Four tablets.
- Microtiter plate, 1x96 wells
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Lactotransferrin (LTF) ELISA Kit
LTF 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Lactotransferrin (LTF) ELISA KitLTF 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Milk, Plasma, Serum, Tissue Homogenate
Lactotransferrin (LTF) ELISA KitLTF 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Milk, Plasma, Serum
Lactotransferrin (LTF) ELISA KitLTF 适用: Cow Colorimetric Competition ELISA 3.12 μg/mL - 200 μg/mL Milk
Lactotransferrin (LTF) ELISA KitLTF 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Milk, Plasma, Saliva, Serum, Tissue Homogenate
Lactotransferrin (LTF) ELISA KitLTF 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Lactotransferrin (LTF) ELISA KitLTF 适用: 人 Colorimetric Sandwich ELISA 3.125 ng/mL - 100 ng/mL Milk, Plasma, Serum, Urine
Lactotransferrin (LTF) ELISA KitLTF 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Lactotransferrin (LTF) ELISA KitLTF 适用: Cow Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Milk, Plasma, Serum, Urine
Lactotransferrin (LTF) ELISA KitLTF 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
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Principle of the Procedure:
- This method is an Enzyme-linked immunosorbent assay (ELISA) for lactoferrin. Lactoferrin is captured by a monoclonal antibody (MAb) that is coated on wells of a sectional microplate. A second LTF-MAb labeled with biotin is added to the well and binds with the captured LTF forming a "sandwich." A solution of streptavidin-peroxidase is then added. Streptavidin has a high affinity for biotin and once bound, its HRP (HRP) label is available for color development by addition of the substrate, o-phenylenediamine (OPD). This color development at 450nm is proportional to the quantity of LTF in the sample.
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C/-20°C
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- Lactoferrin (LTF) (Lactotransferrin (LTF))
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别名
- Lactoferrin
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背景
- Human lactoferrin (LTF) is an 80kD glycoprotein which was first isolated from human milk. LTF is found in most body fluids and secretions, e.g., in the nose, genital tract, and tears.1 In the blood, LTF is secreted by neutrophils and its plasma concentration is positively related to the total pool of neutrophils and to the rate of neutrophil turnover.2 Because of its ability to strongly bind iron, LTF is considered to be bactericidal.3 In a number of cases of inflammation, LTF is released into the extracellular medium from secondary granules of neutrophilic leukocytes.4,5 Its extracellular concentration can therefore be used as an index of neutrophil activation, especially in blood samples containing anti-myeloperoxidase antibodies.
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途径
- Transition Metal Ion Homeostasis
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