Albumin ELISA 试剂盒
Quick Overview for Albumin ELISA 试剂盒 (ABIN5068124)
抗原
See all Albumin (ALB) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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灵敏度
- 5 ng/mL
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产品特性
- Albumin, Human, ELISA Kit (Serum Albumin, ALB, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341)
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组件
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- Microtiter Plate: 1x96 wells
- Control (ng/ml), 1x650ul
- Standard (5ng/ml), 1x650ul
- Standard (10ng/ml), 1x650ul
- Standard (20ng/ml), 1x650ul
- Standard (50ng/ml), 1x650ul
- Standard (100ng/ml), 1x650ul
- HRP Conjugate, 100X, 1x150ul
- Sample Diluent, 20X, 1x10ml
- 0: Wash Solution, 100X, 1x10ml
- 1: TMB Substrate, 1x12ml
- 2: Stop Solution (1% O4), 1x12ml
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板类型
- Pre-coated
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实验流程
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Principle:
- The Human Albumin ELISA kit is based on the binding of human albumin in samples to two antibodies, one immobilized on the microwells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate (TMB) is added and color is developed by the enzymatic reaction of HRP on the substrate, which is directly proportional to the amount of Albumin present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microwell reader. The concentration of albumin in samples and control is calculated from a curve of standards containing known concentrations of albumin.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C
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- Albumin (ALB)
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别名
- Albumin
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途径
- Lipid Metabolism
抗原 See all Albumin (ALB) ELISA试剂盒
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