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HAMA ELISA 试剂盒

This 人 和 小鼠 HAMA ELISA Kit specifically detects HAMA in Serum.
产品编号 ABIN5068122
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for HAMA ELISA 试剂盒 (ABIN5068122)

抗原

See all HAMA products
HAMA (Human Anti-Mouse Antibody (HAMA))

适用

人, 小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Serum
  • 灵敏度

    > 3 ng/mL

    产品特性

    HAMA (Human anti-Mouse Antibody) ELISA Kit

    组件

    • HAMA Microtiter Plate, Pre-coated with mouse IgG. 1x96 wells
    • HRP Conjugate, Mouse IgG labeled with Horseradish Peroxidase (HRP). 1x12ml
    • * HAMA Standard, Rabbit anti-Mouse IgG antibody, supplied as a lyophilized powder from a buffered protein base with preservative. 2x800ng
    • Calibrator Diluent, Animal serum with buffer and preservative. 1x25ml
    • Wash Buffer, 20X, 20-fold concentrated solution of buffered surfactant. 1x60ml
    • Substrate A, Buffered solution with 2. 1x10ml
    • Substrate B, Buffered solution with TMB. 1x10ml
    • Stop Solution, 2N Sulphuric Acid (O4). 1x15ml
  • 板类型

    Pre-coated

    实验流程

    Principle:
    • This assay applies an enzyme-linked immunosorbent assay (ELISA) technique. A purified mouse IgG has been pre-coated onto the wells of microplate. Standard (rabbit anti-mouse IgG) and specimens are added into the appropriate wells simultaneously with a HRP-labelled mouse IgG, and incubated. The Human Anti-Mouse Antibody (HAMA), if present, will bind with the mouse IgG which coated on the wells and HRP- labelled mouse IgG as well, forming a "Sandwich". Following a wash to remove any unbound substance, a substrate solution is added. The developed colour is directly proportional to the anti-mouse antibody concentration in the specimen and Standard samples.

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    4°C/-20°C
  • 抗原 See all HAMA products

    HAMA (Human Anti-Mouse Antibody (HAMA))

    别名

    HAMA (Human anti-Mouse Antibody)

    物质类

    Antibody
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