Triiodothyronine T3 ELISA 试剂盒
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北京 101111
Quick Overview for Triiodothyronine T3 ELISA 试剂盒 (ABIN5068110)
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See all Triiodothyronine T3 (T3) ELISA试剂盒适用
检测方法
应用范围
样品类型
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原理
- Triiodothyronine, Free, Human, ELISA Kit (fT3, Free T3) is based on competitive binding of human free thyroxine from serum samples and enzyme-labeled T3 to T3-specific antibodies immobilized on microtiter well plates. After a washing step, chromogenic substrate is added and color developed. The enzymatic reaction (blue color) is inversely proportional to the amount of T3 present in the sample. The reaction is terminated by adding stopping solution (converts blue to yellow). Absorbance is then measured on a microtiter well ELISA reader at 450nm. and the concentration of free T3 in samples and control is read off the standard curve.
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Analytical Method
- Quantitative
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灵敏度
- 0.3 pg/mL
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产品特性
- Triiodothyronine, Free, Human, ELISA Kit (fT3, Free T3)
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组件
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- Microstrip Plate, 1x96wells
- Assay Buffer, 1x15ml
- Sample Diluent/Standard 0pg/ml 1x10ml
- Standard 2pg/ml 1x500ul
- Standard 4pg/ml 1x500ul
- Standard 8pg/ml 1x500ul
- Standard 16pg/ml 1x500ul
- Standard 40pg/ml 1x500ul
- Low Control (3..5pg/ml), 1x500ul
- 0: High Control (6..5pg/m). 1x500ul
- 1: Ab (HRP), 50X, 1x300ul
- 2: Wash Buffer, 10X, 1x50ml
- 3: TMB Solution, 1x16ml
- 4: Stop Solution (1M sulfuric acid), 1x6ml
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C/-20°C
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- Triiodothyronine T3 (T3)
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别名
- Triiodothyronine
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物质类
- Amino Acid
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