Albumin ELISA 试剂盒
Quick Overview for Albumin ELISA 试剂盒 (ABIN5068089)
抗原
See all Albumin (ALB) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- This Micro- albumin Quantitative using microwell competitive ELISA method provides a convenient, sensitive and specific assay for albumin and free of interference from urine specimens.
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Analytical Method
- Quantitative
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灵敏度
- 250 ng/mL
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产品特性
- Albumin, Human, ELISA Kit (Serum Albumin, ALB, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341)
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组件
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- Human Albumin coated microwell strip plate: 1 X 96 wells
- Albumin Std. A: 1 X 11ml
- Albumin Std. B (2.5ug/ml): 1 X 0.5ml
- Albumin Std. C (5ug/ml): 1 X 0.5ml
- Albumin Std. D (25ug/ml): 1 X 0.5ml
- Albumin Std. E (50ug/ml): 1 X 0.5ml
- Albumin Std. F (100ug/ml): 1 X 0.5ml
- Albumin Control (exact value printed on the vial)
- Anti-Albumin-HRP Conjugate: 1 X 11ml
- HRP substrate Solution: 1 X 11ml
- Wash buffer (100X): 1 X 10ml, dilute 1:100 with distilled water
- Stop solution (ready-to-use): 1 X 10ml
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Albumin (ALB) ELISA Kit
ALB 适用: 人 Colorimetric Competition ELISA 246.9 ng/mL - 20000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Albumin (ALB) ELISA KitALB 适用: 小鼠 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Competition ELISA 123.5 ng/mL - 10000 ng/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 3.13 μg/mL - 200 μg/mL Cell Culture Supernatant, Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 7.813 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Albumin (ALB) ELISA KitHigh Sensitivity ALB 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Albumin (ALB) ELISA KitALB 适用: 犬 Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
Albumin (ALB) ELISA KitALB 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
Albumin (ALB) ELISA KitALB 适用: 兔 Colorimetric Competition ELISA 617.3 ng/mL - 50000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
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Principle:
- Albumin ELISA kit is based on competitive binding of human albumin from urine samples and albumin coated on the microwell plate to the enzyme labeled anti- human albumin antibody. Higher concentrations of albumin in the urine samples result in decreased binding of enzyme (HRP) labeled antibody to the microwell plate. After a washing step, chromogenic substrate is added and color developed. The enzymatic reaction (color) is inversely proportional to the amount of albumin present in the sample. Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450nm. and the concentration of albumin in samples and control is read off the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C
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- Albumin (ALB)
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别名
- Albumin
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背景
- The analytical determination of the protein albumin in urine is important because increased values indicate an increased risk of developing end-stage renal diseases and cardiovascular disease among people with diabetes. Also albumin in urine is a sensitive indicator of renal damage caused by exposure to nephrotoxic substances. The most significant and well-documented of these abnormalities is a subtle increase in the urinary albumin excretion rate, known as mico-albuminuria. Mico-albuminuria is not measurable by conventional techniques for detecting proteinuria. It is believed that mico-albuminuria represents a reversible stage of renal dysfunction, whereas overt proteinuria reflects irreversible disease. Proteinuria typically appears about twenty years after the onset of diabetes, whereas mico-albuminuria can be detected within the first ten years. Mico-albuminuria (30-150 μg/min) has been established as a marker predictive of subsequent development of diabetic nephropathy. Periodic monitoring(2-3 times/year) of urinary of albumin levels in the diabetic patient is therefore recommended so that the initial escalation of renal damage can be detected and appropriate treatment regimens can be instituted. Radial immunodiffusion, immunoturbidimetric, immunophelometric method and RIA have been used for the albumin assay in urine.
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途径
- Lipid Metabolism
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