Leptin ELISA 试剂盒
Quick Overview for Leptin ELISA 试剂盒 (ABIN5068068)
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检测方法
实验类型
应用范围
样品类型
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原理
- This Human Leptin ELISA kit is to be used for the in vitro quantitative determination of leptin in human serum, plasma, buffered solution or cell culture medium. The assay recognizes both natural and recombinant forms of leptin.
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Analytical Method
- Quantitative
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交叉反应 （详细）
- Calculated cross reactivity: Hu
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灵敏度
- > 3.5 pg/mL
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产品特性
- Leptin, Human (Ob, Obese Protein, Obesity Factor) ELISA Kit
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组件
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- 96 Test Kit 192 Test Kit
- Standard &
- 25,&
- 25, 2 vials 4 vials
- Standard Diluent 1x60ml 2x60ml
- Microtiter Plate 1x96wells 2x96wells
- Mab (Biotin) 1x11ml 2x11ml
- Streptavidin (HRP), 100X 1x125ul 2x125ul
- HRP Diluent 1x25ml 1x25ml
- Wash Buffer, 25X 1x100ml 1x100ml
- TMB 1x25ml 1x25ml
- Stop Solution 1x25ml 1x25ml
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Leptin (LEP) ELISA Kit
LEP 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 人 Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 兔 Colorimetric Competition ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 兔 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: 小鸡 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP 适用: Salmon Colorimetric Competition ELISA 62.5 pg/mL - 1000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP 适用: Pig Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP 适用: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
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Principle:
- This Human Leptin kit is a solid phase sandwich ELISA. A monoclonal antibody specific for human leptin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known leptin content, control specimens, and unknowns, are pipetted into these wells followed by addition of a second biotinylated monoclonal antibody. During the first incubation, the leptin antigen binds to the immobilized (capture) antibody on one site and to the solution phase biotinylated antibody on a second site. After removal of excess secondary antibody, Streptavidin-Peroxidase enzyme is added. This binds to the biotinylated antibody to complete the four-member sandwich. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of leptin present in the original specimen.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 4°C
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- Leptin (LEP)
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别名
- Leptin
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途径
- JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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