IL1RL1 ELISA 试剂盒
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北京 101111
Quick Overview for IL1RL1 ELISA 试剂盒 (ABIN5068026)
抗原
See all IL1RL1 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- The ST2, Mouse ELISA Kit is a 4.5 hour solid phase ELISA designed to measure mouse ST2 in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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灵敏度
- 0.014 ng/mL
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产品特性
- ST2, Mouse ELISA Kit (Interleukin-1 Receptor-like 1, Protein ST2, IL1RL1, DER4, T1)
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组件
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- Microwell Strips, 1x96 wells
- * Standard (lyophilized), 1x1vial (50ng)
- * Control (lyophilized), 0..62ng/ml, 1x1vial
- HRP Conjugate, 1x12ml
- Assay Diluent, 1x12ml
- Calibrator Diluent, 1x21ml
- Wash Buffer, 25X, 1x50ml
- Color Reagent A, 1x12ml
- Color Reagent B, 1x12ml
- 0: Stop Solution (HCl), 1x23ml
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板类型
- Pre-coated
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实验流程
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Principle:
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse ST2 has been pre-coated onto a microplate. Standards, Control, and samples are pipetted into the wells and any mouse ST2 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for mouse ST2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells. The enzyme reaction yields a blue product that turns yellow when the Stop Solution is added. The intensity of the color measured is in proportion to the amount of mouse ST2 bound in the initial step. The sample values are then read off the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C/-20°C
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- IL1RL1 (Interleukin 1 Receptor-Like 1 (IL1RL1))
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别名
- ST2
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