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Albumin ELISA 试剂盒

This 小鼠 Albumin ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 Albumin.
产品编号 ABIN5067986
发货至: 中国

Quick Overview for Albumin ELISA 试剂盒 (ABIN5067986)

抗原

See all Albumin (ALB) ELISA试剂盒
Albumin (ALB)

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Culture Cells, Saliva, Tissue Samples
  • 原理

    The Mouse Albumin ELISA Kit is an in vitro immunoassay for the quantification of albumin circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of mouse cells.

    Analytical Method

    Quantitative

    交叉反应 (详细)

    Calculated cross reactivity: Mo

    灵敏度

    10 ng/mL

    产品特性

    Albumin, Mouse, ELISA Kit

    组件

    • Microtiter Plate: 1x96 wells
    • Control (ng/ml), 1x650ul
    • Standard (20ng/ml), 1x650ul
    • Standard (50ng/ml), 1x650ul
    • Standard (100ng/ml), 1x650ul
    • Standard (200ng/ml), 1x650ul
    • Standard (400ng/ml), 1x650ul
    • HRP Conjugate (100X), 1x150ul
    • Sample Diluent (20X), 1x10ml
    • 0: Wash Solution (100X), 1x10ml
    • 1: TMB Substrate, 1x12ml
    • 2: Stop Solution, 1x12ml
  • 实验流程

    Principle:
    • The Mouse Albumin ELISA kit is based on the binding of mouse albumin in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of albumin present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of albumin in samples and control is calculated from a curve of standards containing known concentrations of albumin.

    限制

    仅限研究用
  • 储存条件

    4 °C

    储存方法

    4°C
  • 抗原 See all Albumin (ALB) ELISA试剂盒

    Albumin (ALB)

    别名

    Albumin

    途径

    Lipid Metabolism
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