Albumin ELISA 试剂盒
Quick Overview for Albumin ELISA 试剂盒 (ABIN5067986)
抗原
See all Albumin (ALB) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- The Mouse Albumin ELISA Kit is an in vitro immunoassay for the quantification of albumin circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of mouse cells.
-
Analytical Method
- Quantitative
-
交叉反应 (详细)
- Calculated cross reactivity: Mo
-
灵敏度
- 10 ng/mL
-
产品特性
- Albumin, Mouse, ELISA Kit
-
组件
-
- Microtiter Plate: 1x96 wells
- Control (ng/ml), 1x650ul
- Standard (20ng/ml), 1x650ul
- Standard (50ng/ml), 1x650ul
- Standard (100ng/ml), 1x650ul
- Standard (200ng/ml), 1x650ul
- Standard (400ng/ml), 1x650ul
- HRP Conjugate (100X), 1x150ul
- Sample Diluent (20X), 1x10ml
- 0: Wash Solution (100X), 1x10ml
- 1: TMB Substrate, 1x12ml
- 2: Stop Solution, 1x12ml
-
-
-
-
实验流程
-
Principle:
- The Mouse Albumin ELISA kit is based on the binding of mouse albumin in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of albumin present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of albumin in samples and control is calculated from a curve of standards containing known concentrations of albumin.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
储存方法
- 4°C
-
-
- Albumin (ALB)
-
别名
- Albumin
-
途径
- Lipid Metabolism
抗原 See all Albumin (ALB) ELISA试剂盒
-