Kynurenine ELISA 试剂盒
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北京 101111
Quick Overview for Kynurenine ELISA 试剂盒 (ABIN5067984)
抗原
See all Kynurenine products适用
检测方法
实验类型
应用范围
样品类型
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原理
- The Kynurenine (KYN) ELISA Kit is a quantitative competitive assay for the detection of KYN in human serum, plasma, tissue homogenates and other biological fluids.
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Analytical Method
- Quantitative
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灵敏度
- > 4.69 pM/mL
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产品特性
- Kynurenine (KYN) ELISA Kit (Human)
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组件
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- *356970A: Microtiter Strips, 1x96 wells (8x12 wells).
- *356970B: Standard, 2x1 vial
- 356970C: Sample/Standard Dilution Buffer, 1x20ml
- 356970D: Antibody (Biotin) (Concentrated), 1x60ul
- 356970E: Antibody Dilution Buffer, 1x10ml
- 356970F: Streptavidin (HRP) (SABC), 1x120ul
- 356970G: SABC Dilution Buffer, 1x10ml
- 356970H: TMB Substrate, 1x10ml
- 356970J: Stop Solution, 1x10ml
- 356970K: Wash Buffer, 25X, 1x30ml
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Kynurenine ELISA Kit
适用: 人 Colorimetric Competition ELISA 7.813 pmol/mL - 500 pmol/mL Plasma, Serum, Tissue Homogenate
Kynurenine ELISA Kit适用: 小鼠 Colorimetric Competition ELISA 7.813 pmol/mL - 500 pmol/mL Plasma, Serum, Tissue Homogenate
Kynurenine ELISA Kit适用: Various Species Colorimetric Competition ELISA 7.813 pmol/mL - 500 pmol/mL Plasma, Serum, Tissue Homogenate
Kynurenine ELISA Kit适用: 猴 Colorimetric Competition ELISA 7.813 pmol/mL - 500 pmol/mL Plasma, Serum, Tissue Homogenate
Kynurenine ELISA Kit适用: 人 Colorimetric Competition ELISA 0.12-500 pM/mL Serum, Tissue Homogenate, Urine
Kynurenine ELISA Kit适用: 犬 Colorimetric Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
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Principle:
- The microtiter plate provided in this kit has been pre-coated with KYN. KYN in the standard or sample competes with a fixed amount of plate-coated KYN for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of KYN in the samples is determined by comparing the absorbance of the samples to the standard curve.
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实验精密度
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Precision:
- Intra-Assay CV: <8%
- Inter-Assay CV: <10%
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C/-20°C
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- Kynurenine
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别名
- Kynurenine (KYN)
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物质类
- Chemical
抗原 See all Kynurenine products
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